摘要
目的 探讨超声消融术对癌细胞超微结构和细胞周期的影响。 方法 体外超声消融 ( 42kHz, 0. 22W)Walker-256细胞,扫描电镜、透射电镜和流式细胞仪分别观察细胞变化。 结果 超声消融Walker-256细胞后,电镜观察发现:细胞膜破裂、细胞器紊乱不清、核染色质碎裂,提示细胞已受到严重的损伤。流式细胞仪检查发现,随着超声剂量的增大,细胞S期下降,G2 +M期增加,说明细胞合成抑制,放射敏感性增加。 结论 在细胞水平验证了超声消融术对癌细胞具有杀伤效应。
Objective To observe changes of the ultrastructure and the cell cycle of hepatoma cells exposed to ultrasound-mediated disruption. Methods Suspensions of Walker-256 hepatoma in vitro exposed to ultrasound disruption with the acoustic power output 0 22 W and the ultrasound frequency 42 kHz were observed by the scanning electron microscopy, the transmission electron microscopy and the flow cytometric analysis of DNA content. Results Cells after ultrasound disruption revealed by scanning electron microscopy exhibited ruptured membrance, disordered organellae and disrupted nucleus, showing typical severe destruction of the cells. Flow cytometric analysis of DNA content demonstrated an increased in the population of cells in the G 2 and M phases of the cell cycle but a decrease in number of cells in the S phase, suggesting an inhibition of the cell synthese and an increased sensitivity to radiation. Conclusions Ultrasound disruption can destroy tumor cells effectively at the cellular level.
出处
《中国微创外科杂志》
CSCD
2005年第2期119-121,共3页
Chinese Journal of Minimally Invasive Surgery
