摘要
目的探讨血清高迁移率蛋白-1（HMGB1）和高敏C反应蛋白（Hs—CRP）的含量变化在急性阑尾炎诊断中的价值。方法采用酶联免疫吸附法（ELISA）检测血清HMGB1含量，同时采用免疫比浊法对血清Hs—CRP进行测定；将二三组阑尾炎患者（急性坏疽性阑尾炎35例，急性化脓性阑尾炎48例，急性单纯性阑尾炎32例）和阑尾无炎症患者（30例）HMGBI和Hs-CRP水平进行比较。结果三组急性阑尾炎患者与阑尾无炎症患者比较，HMGB1和Hs—CRP水平均较阑尾无炎症患者明显升高（P〈0．01）；急性坏疽性阑尾炎及急性化脓性阑尾炎与急性单纯性阑尾炎比较，HMGB1和Hs-CRP水平明显升高（P〈0．01）。同时急性阑尾炎患者HMGB1浓度与Hs—CRP浓度呈正相关（r=0．62，P〈0．01）。结论在临床症状及白细胞计数升高的基础上，结合血清HMGB1和Hs—CRP的测定对急性阑尾炎的诊断具有一定价值．
Objective To analyze the value of high mobility groupbox-1 （ HMGB1 ） and high-sensitive C-reactive Protein（ Hs-CRP）in the diagnosis of acute appendicitis. Methods The HMGB1 in serum of no inflammation cases and three groups of acute appendicitis was examined respectively. Concentrations of HMGB1 was determined by enzyme linked immunosorbent assay（ELISA） , and Hs-CRP was determined by rate nephe- lometry immunoassay. Results The serum HMGB1 and Hs-CRP level in three groups of acute appendicitis was much higher than that in the no inflammation cases with a significant difference（ P 〈 0. 01 ） ; The serum HMGBI and Hs-CRP level in patients with suppurative and gangrenous appendicitis were much higher than that in patients with simple appendicitis（ P 〈 0. 01 ）. There was positive correlation between the concentrations of HMGB1 and hs-CRP（r =0. 62,P 〈0. 01 ）. Conclusion The serum HMGB1 and Hs-CRP test are valuable in diagnosis of acute appendicitis on the basis of the clinical symptom and WBC levels.
出处
《中国临床实用医学》
2010年第5期69-71,共3页
China Clinical Practical Medicine
关键词
阑尾炎
高迁移率蛋白-1
高敏C反应蛋白
Appendicitis
High mobility groupbox-1 （ HMGB1 ）
High-sensitive C-reactive Protein （Hs-CRP）
