摘要
目的 测定心肌缺血再灌注过程中X染色体连锁凋亡抑制蛋白（XIAP）动态表达变化,探讨其与缺血再灌注损伤所致心肌细胞凋亡的关系.方法 将健康成年雄性SD大鼠随机分为两组,手术组和伪手术组,每组根据再灌注时间再分为6组：缺血30min,再灌注0、1、2、3、12、24 h组.采用半胱天冬酶-3（Caapaae-3）活性检测判定大鼠心肌细胞凋亡发生情况;用Western Blot和实时定量PCR技术分别检测各组心肌组织中XIAP的蛋白和基因表达水平.结果 Caspase-3活性从心肌缺血再灌注1 h开始升高,再灌注12 h活性最大,再灌注24 h趋于正常.心肌缺血再灌注3 h,XIAP蛋白表达开始降低,再灌注12 h表达最低;而XIAP的mRNA水平表达各组差异无统计学意义.结论 成年大鼠心肌缺血再灌注后Caspase-3活性增高可能与XIAP表达降低有关,提示XIAP表达降低可能参与了心肌缺血再灌注诱导的细胞凋亡.
Objective To detect the time course of XIAP expression after myocardial ischemia/reperfu- sion, and investigate the relationship between XIAP expression and myocardial apoptosis induced by ischemia/ reperfusion injury. Methods Male Sprague-Dawley adult rats were randomly divided into two groups： ischemia/ reperfusion group and sham group. Every group was assigned to six parts by different reperfusion hours： ischemia 30 min and reperfusion 0, 1, 2, 3, 12 and 24 hours. Myocardial apoptosis was analyzed by Caspase-3 activity assay. The protein and mRNA expression of XIAP were determined respectively by Western Blot and Real Time PCR. Results Caspase-3 activity ratio began to increase at 1 hour after reperfnsion, and reached the peak at 12 hour after reperfusion, and returned to normal at 24 hour after reperfusion. The protein expression of XIAP began to decrease at 3 hour after reperfusion, and reached the minimum at 12 hour after reperfusion. There was no sta- tistical difference in mRNA expression of XIAP between every group. Conclusion The increase of Caspase-3 ac- tivity is related to XIAP down-regulation after myocardial ischemia/reperfusion, which suggested XIAP down-reg- ulation might be involved in myocardial apoptosis induced by isehemia/reperfusion injury.
出处
《中国心血管病研究》
CAS
2010年第5期376-379,共4页
Chinese Journal of Cardiovascular Research
基金
太原市大学生创新创业项目（091222020）
