摘要
目的：研究异种表面角膜镜片术后机体对异种角膜的免疫反应，阐明人体对异种角膜的免疫耐受机制。方法：应用流式细胞仪对９例（９只眼）接受异种表面角膜镜片术患者术后外周血Ｔ细胞的分布和自然杀伤细胞的活性进行了分析。结果：术后２个月，Ｔ细胞未被激活，ＮＫ活化程度显著提高（Ｐ＜００１），但未激发排斥反应。结论：猴角膜镜片虽可诱发轻微的免疫应答，但未达到增强抗原诱发的Ｔ细胞增殖应答的程度。可能与猴和人的种属相近，角膜镜片的抗原性低，抗原不易或缓慢被吞噬细胞摄取而成为致耐原，进而诱导免疫耐受有关。术后局部应用免疫抑制剂可防止移植排斥反应的发生。
To study the mechanism of the immune tolerance of the xenogenic epikeratophakia,4 patients with keratoconus and 5 patients with aphakia were treated by epikeratophakia monkey lenticules The peripheral lymphocyte subsets and the natural killer cell activity were analysed with flow cytometry before and 1 month,2 months after surgery respectively T cell was not activated and the rejection was not set off though the activation of NK was significant 2 months postoperatively The immune response activated by monkey lenticules was slight and not enough to promote the proliferation of the lenticule induced T cell It was possible that the low antigenicity of monkey lenticules could progressively cause the immune tolerance,and topical steroid may be helpful to inhibit the xenogenic rejection
出处
《眼科》
CAS
1999年第1期48-51,共4页
Ophthalmology in China
关键词
表面角膜镜片术
T淋巴细胞
杀伤细胞
流式细胞
Epikeratophakia
Transplantation,heterologous
T Lymphocytes
Killer cells,natural
Immune tolerance
Flow cytometry
