摘要
目的:观察抗纤复方对大鼠肝贮脂细胞产生TGFβ_1,的影响。方法:采用血清药理学方法处理贮脂细胞,以招肺上皮细胞(MvlLu)生长抑制MTT法检测培养上清液中TG即活性,免疫细胞化学染色检测贮脂细胞TGFβ_1的生物活性和细胞内TGFβ_1的表达。结果:经抗纤复方处理的贮脂细胞上清液中TGFβ_1的生物活性和细胞内TGFβ_1的表达受到显著抑制,其中对纤维肝贮脂细胞的作用更明显,抑制作用优于秋水仙碱。结论:抗纤复方能抑制贮脂细胞产生TGFβ_1,阻断肝纤维化时贮脂细胞合成胶原等ECM的自分泌放大过程。
Aims: To observe of Anti-fibrosis Compond Prescription (ACP) on the expression of transforming growth factor β1 (TGF β1) in rat liver fat-storing cells (FSC). Methods: The serum pharmacology mehhod was employed to treat the FSC. and the TGFβ1 activity in the srpernatant of culture was measured by mink lung epihtilisl cell ( MvlLu) proliferation inhibition MTT assay, and observe the espression of TGF β1 in FSC, Results: The bioactivity of TGFβ1, and the cytoplasmic expression of FSC were evidently inhibited in the culture supernatant pretreated with ACP, and amore significant inhihitive effect was found in the fibrous FSC. Conclusions: ACP may inhibit the production of TGF β1 in FSC, and thus, block the FSC autocrine magnification process and reduce the synthesisof ECM such as collagen, etc during liver fibrosis.
出处
《中西医结合肝病杂志》
CAS
1999年第1期21-23,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
关键词
贮脂细胞
TGFΒ1
肝纤维化
抗纤复方
Fat-storing Cell TGFβ1 Liver Fibrosis Anti-fibrosis Compond Prescription
