摘要
目的:观察10种黄酮类化合物对糖尿病致病机制中4条重要通路的多靶点抑制作用,发挥中药多靶点、综合治疗的优势。方法:从正常大鼠脑组织中提取半纯化的蛋白激酶C(PKC)、肾脏组织中提取醛糖还原酶(AR)粗酶,Fenton试剂体外激发正常大鼠脑组织脂质过氧化生成丙二醛(MDA)及体外合成高级糖化终产物(AGEs),将上述酶或合成物与10种黄酮类化合物(终浓度为1 mg.mL-1)作用,观察黄酮类化合物对上述酶或合成物的抑制作用。结果:黄芩苷和灯盏花素对AR,PKC,AGEs及MDA均有不同程度的抑制作用。结论:上述结果提示此两种黄酮类化合物可以通过抑制上述4条通路来预防糖尿病及其并发症的发生,为进一步的机制研究及临床应用提供良好的实验依据。
Objective：To estimate the inhibitory effects of 10 flavonoid glycosides on the 4 important pathways in diabetic pathogenesis,and to find new and effective extracts of Chinese herbs for treatment of diabetes.Methods：Semi-purified protein kinase C（PKC） was extracted from brains of normal rats,and crude aldose reductase（AR） was from kidneys.Malondialdehyde（MDA） was produced by activating brain tissue with Fenton reagent.Advanced glycation end products（AGEs） were synthetized in vitro.The effects of flavonoid glycosides（1 mg·mL^-1） on the enzymes or their products were observed.Results：Baicalin and breviscapine showed inhibitory effects on AR,PKC,AGEs and MDA with different degrees of activity.Conclusion：Among 10 flavonoid glycosides,baicalin and breviscapine have potentially preventing effects for diabetic pathogenesis by inhibiting the 4 important pathways.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2010年第9期793-796,共4页
Chinese Journal of New Drugs
基金
浙江省中医药管理局青年基金(2007YA022)
关键词
活性黄酮
糖尿病
并发症
active flavanoid
diabetes
complication
