摘要
目的:探讨EZH2蛋白及其mRNA在前列腺癌中的表达以及两者与临床病理参数间的关系。方法:通过组织芯片技术,运用免疫组化(EnVision法)和原位杂交方法分别检测48例前列腺癌中EZH2蛋白及其mRNA的表达,同时检测常规石蜡组织中15例良性前列腺增生组织和12例高级别上皮内瘤变组织中的EZH2蛋白及其mRNA的表达作为对照。结果:EZH2蛋白和mRNA在前列腺癌中的阳性率(87.5%、81.25%)明显高于良性前列腺增生组织(13.33%、6.67%)和高级别上皮内瘤(16.67%、16.67%),差异有统计学意义(P<0.05)。免疫组化显示,EZH2蛋白在Gleason≥7分组阳性表达率(96.67%)明显高于Gleason≤6分组(72.22%),差异有统计学意义(P<0.05)。EZH2蛋白阳性表达率与TNM分期比较,T3～T4期(100%)明显高于T1～T2期(76.92%),亦有统计学意义(P<0.05),而EZH2蛋白表达与年龄和PSA无关(P>0.05)。原位杂交显示,EZH2mRNA的阳性表达率T3～T4期(100%)高于T1～T2期(85%),与TNM分期显著相关(P<0.05),而与年龄、PSA和Gleason分级无关(P>0.05)。分段评价预后估计较好组与预后估计较差组,两组之间EZH2蛋白及其mRNA表达阳性率均有显著性差异(P<0.05)。结论:EZH2蛋白及其mRNA在前列腺癌中高表达,提示其在前列腺癌的发生、发展过程中可能起重要作用,有可能成为判定前列腺癌恶性程度进程和预后的参考指标。
Objective：To investigate the expressions of the EZH2 protein and EZH2 mRNA in human prostate cancer（PCa） and their correlation with the clinicopathologic parameters.Methods：A tissue microarray（TMA） was constructed,which contained 48 dots of formalin-fixed paraffin-embedded tissue samples of human PCa.The expressions of the EZH2 protein and EZH2 mRNA in the samples were detected by immunohistochemistry（EnVision） and in situ hybridization（ISH）.Another 15 cases of human benign prostate hyperplasia（BPH） and 12 cases of human prostate intraepithelial neoplasia（HGPIN） were taken as controls.Results：The positive rates of the EZH2 protein and mRNA were significantly higher in PCa than in BPH and HGPIN（87.5%vs 13.33%and 16.67%,81.25%vs 6.67%and 16.67%,P〈0.05）.The positive expression of the EZH2 protein was 96.67%and 72.22%in the Gleason score≥7 and Gleason score≤6 groups,respectively,with significant differences between the two groups（P〈0.05）. The positivity of the EZH2 protein was significantly related to the TNM stage,increasing with tumor progression（P〈0.05）,but not to age and serum PSA（P〈0.05）,and so was that of EZH2 mRNA to TNM stage（P〈0.05）,but not to age,serum PSA and Gleason score（P〈0.05）.When the above characteristics were regarded as two-level discrete variables,both the EZH2 protein and EZH2 mRNA showed statistically significant differences in the positive expression rate（P〈0.05）.Conclusion：The over-expressions of the EZH2 protein and EZH2 mRNA may play an important role in the pathogenesis and progression of PCa and provide some reference indexes for estimating the malignancy,progression and prognosis of PCa.
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2010年第2期123-128,共6页
National Journal of Andrology
关键词
前列腺肿瘤
EZH2
组织芯片
免疫组化
原位杂交
prostatic neoplasm
EZH2
tissue microarray
immunohistochemistry
in situ hybridization
