摘要
通过摇瓶培养考察金属离子(Mg2+、Ca2+、Fe3+、Zn2+、Mn2+、Cu2+)对斯达油脂酵母(Lipomyces starkeyi 1809)生长和发酵生产油脂的影响。结果表明:在不同金属离子的各种浓度下,斯达油脂酵母的菌体生物量、油脂产量、油脂含量和油脂系数的差异均达到极显著水平(P<0.01)。当培养基中缺失Mg2+或Fe3+或Zn2+时明显抑制菌体生长和油脂积累,添加适当浓度的Mg2+、Fe3+、Zn2+可促进斯达油脂酵母菌体生长和油脂合成,菌体利用底物合成油脂的转化率显著提高;添加适当浓度的Ca2+或Cu2+有利于菌体生长和油脂积累;Mn2+缺失有利于菌体生长和油脂合成,Mn2+浓度越高越不利于油脂积累。在试验浓度范围,MgSO4.7H2O 1 000 mg/L、CaCl2.2H2O 500 mg/L、FeCl3.6 H2O 1.6 mg/L、ZnSO4.7H2O 0.3 mg/L、MnCl2.4H2O 0.008 mg/L、CuCl2.2H2O 0.08 mg/L为斯达油脂酵母生长和油脂积累的适宜浓度。结论:在培养基C/N一定的条件下,金属离子对斯达油脂酵母生长和产油脂能力起着重要的作用,本试验结果对建立微生物油脂发酵调控的新策略具有重要的理论和实践意义。
Effect of metal ions（Mg2＋,Ca2＋,Fe3＋,Zn2＋,Mn2＋,Cu2＋） on cell growth and lipid production with Lipomyces starkeyi 1809 was investigated by fermentating in shaking flask.results was revealed that when added different metal ions at different concentration,the variance of biomass,lipid yield,lipid content and lipid coefficient reached a significant level（P0.01）.Absence of Mg2＋ or Fe3＋ or Zn2＋ in culture medium could significantly inhibited cell growth and lipid synthesis,whereas supplementing them at suitable concentration promoted cell growth and lipid synthesis,microorganism could make use of substrate to synthesize lipid,and the conversion rate was improved significantly.Supplementing Ca2＋ or Cu2＋ at proper concentration would facilitate cell growth and lipid biosynthesis.Absence of Mn2＋ conduced to cell growth and lipid accumulation,the higher concentration of Mn2＋ was unfavorable to lipid accumulation.Within the range of experimental concentration,the suitable concentration of metal ions for cell growth and lipid accumulation were as follows： MgSO4.7H2O 1 000 mg/L,CaCl2.2H2O 500 mg/L,FeCl3.6H2O 1.6 mg/L,ZnSO4.7H2O 0.3 mg/L,MnCl2.4H2O 0.008 mg/L,CuCl2.2H2O 0.08 mg/L.Conclusion： when the C/N was unaltered in culture medium,metal ions play an important role in cell growth and lipid production,the result has an important academic and practical meaning to establish a new strategy of microbial oil fermentation control.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2010年第2期67-74,共8页
Journal of Chinese Institute Of Food Science and Technology
基金
河南省基础与前沿技术研究项目(082300440110)
