摘要
以一株酵母菌为产油菌株,研究了在1 L发酵罐中发酵油脂的工艺条件,以苏丹黑染色法定量分析油脂,并对发酵油脂组成进行了气相色谱-质谱联用分析。结果表明:发酵罐最佳培养条件为30°C,通气量1.1 vvm,pH=6,转速300 r/min。分析表明发酵油脂中含有多种不饱和脂肪酸,其中软脂酸17.80%、硬脂酸10.27%、油酸53.02%、亚油酸5.29%,这与植物油组成相似。同时以微生物发酵油脂为原料,初步研究了碱催化制备生物柴油。
A kind of microzyme was used in lipid production,and the effects of operation conditions in 1 L fermentor culture were studied.The quantitative test lipid by Sudan Black B straining and confirmed the component of lipid by gas chromatograph and mass spectrum(GC-MS) were carried out.The results showed that the optimal operation conditions in 1 L fermentor culture were as follows:at 30°C,1.1 vvm of ventilation,pH=6,and 300 r/min of stirrer speed.The analysis indicated that the components of lipid produced by fermentation were mainly made of palmitic acid,steric acid,oleic acid and linoleic acid,which were 17.80%,10.27%,53.02%,and 5.29%,respectively,and the components and their portions are very like those in vegetable oil.Furthermore the lipid produced by fermentation was used as raw material to produce biodiesel with alkali as catalyst.
出处
《现代化工》
CAS
CSCD
北大核心
2006年第z2期279-280,282,共3页
Modern Chemical Industry
基金
"211"项目资助
关键词
微生物
发酵
油脂
生物柴油
microorganism
fermentation
lipid
biodiesel
