摘要
目的探讨血管内皮生长因子（VEGF）1498C／T、936C／T基因多态性与结直肠腺瘤易感性的关系。方法对224份结直肠腺瘤及200份正常对照标本进行研究．应用TaqMa。探针法检测VEGF1498C／T、936C／T基因型，搜集相对应的临床病理资料。结果与VEGF936CC基因型相比．CT基因型和CT＋TT基因型患者结直肠腺瘤的发生危险显著增加（DR=2．00，95％CI：1．23—3．25，P=0．006；OR=2．04，95％C1：1．28—3．26，P=0．003）；与VEGF936-C等位基因相比，T等位基因携带者结直肠腺瘤的发生危险亦显著增加（DR=1．91，95％CI：1．25—2．91．P=0．003）。结直肠腺瘤患者为VEGF936CT＋TT基因型或携带T等位基因，其病理类型倾向于绒毛状腺瘤（OR=2．54，95％CI：1．12—5．75，P：0．040；OR=3．08，95％CI：1．64-5．80，P=0．001）。VEGF1498C／T基因多态性在腺瘤组与对照组间的差异无统计学意义（19〉0．05）。结论VEGF936C／T基凶多态性与结直肠腺瘤易感性密切相关。
Objective To examine the association between polymorphism of vascular endothelial growth factor （VEGF）1498 C/T,936 C/T and colorectal adenoma genetic susceptibility. Methods A case-control study of 224 colorectal adenomas and 200 controls was conducted and VEGF genotypes were determined based on TaqMan-probe assay. The epidemiological factors were collected through questionnaire. Accordingly, the clinicopathological data of each sample were also investigated. Results The carriage of 936 CT and CT＋TF genotypes had significantly higher risk of colorectal adenoma （CT vs. CC, OR=2.00, 95% CI： 1.23-3.25, P=0.006; CT＋TF vs. CC, OR=2.04, 95% CI： 1.28-3.26, P= 0.003）. 936-T allele carriage had increased risk of colorectal adenoma （OR=1.91, 95% CI： 1.25-2.91, P=0.003）. The genotypes of 1498 C/T and the frequency of C/T allele showed no differences between healthy persons and patients（P〉0.05）. In patients with 936 CT＋TT and 936-T allele implied a tendency of villous adenoma category （CT＋TF vs. CC, OR=2.54, 95% CI： 1.12-5.75, P=0.040; T allele vs. C allele, 0R=3.08, 95% CI, 1.64-5.80,P=0.00 1）. Conclusion VEGF 936 C/T polymorphism can influence susceptibility to colorectal adenoma.
出处
《中华胃肠外科杂志》
CAS
北大核心
2010年第4期286-288,共3页
Chinese Journal of Gastrointestinal Surgery
基金
湖北省“十一五”重大科技攻关项目（2006AA301A05）
关键词
结直肠肿瘤
腺瘤
血管内皮生长因子
多态性
单核苷酸
Colorectal neoplasms
Adenoma
Vascular endothelial growth factor
Polymorphism, single nucleotide
