摘要
为探明自由基引起红细胞流变性改变的作用机理，我们对体外自由基作用三十分钟前后红细胞膜剪切弹性模量、细胞表面指数、膜脂质分子和蛋白分子流动性进行了比较分析。发现自由基作用后，红细胞膜剪切弹性模量、膜脂质分子和蛋白分子的相对旋转时间分别增加了４９．８％、４９．５％和１９１．４％，而红细胞表面指数无变化；此外，随着自由基浓度的降低以上变化也随之减轻；在自由基损伤后的第三小时，上述改变部分回复。本研究说明，自由基引起脂质和蛋白分子流动性降低共同导致红细胞膜剪切弹性模量的增加，并且一定浓度自由基对红细胞流变性的损伤是部分可逆的。
n order to investigate the effects of free radicals on RBCs rheology and the molecular fluidity of RBCs membrane, after reactions with free radical system, membrane elastic modulus, surface index of cells, rotational correlation times of lipids and proteins of RBCs membrane were studied in vitro. Results showed that all of the membrane shear elastic modulus, rotational correlation times of the membrane lipids and proteins were significantly increased by 49.8%, 49.5% and 191.4% separately after free radical attacking, but no influence on cell volume was found due to unchanged cells surface index. In the present study, the changes of rheological parameters were concentration depend on free radicals, and partly recovered three hours after damage. All the above demonstrated that the immobilities of both lipids and proteins resulted from free radical damage contributed together to disfluidity of RBCs membrane.
出处
《中国生物医学工程学报》
EI
CAS
CSCD
北大核心
1998年第1期65-71,共7页
Chinese Journal of Biomedical Engineering
基金
国家自然科学基金
北京市自然科学基金
关键词
自由基
红细胞膜
剪切弹性模量
膜脂质分子
流动
Free radical
RBCs membrane shear elastic modulus
Molecular fluidities of membrane Iipids and proteins
