摘要
目的探讨仪器液体比例法（BacT／ALERT 3D与MGIT960系统）与L—J比例法测试结核分枝杆菌药物敏感性结果的一致性。方法用BacT/ALERT3D快速药敏法、MGIT960快速药敏法、L—J比例法同时对107株结核分枝杆菌进行INH、SM、RFP及EMB四种抗结核药物的耐药性测定。结果（1）INH、SM、RFP及EMB用BacT／ALERT3D法、MGIT960法、L—J比例法药敏测试结果一致率分别为：93％、90％、95％、90％。（2）BacT／ALERT3D法、MGIT960法、L—J比例法对INH、SM、RFP、EMB的耐药率分别是：27％、28％、24％；12％、13％、19％；25％、25％、22％；7％、6％、9％。三种方法四种药物的耐药性均无显著性差异（P〉0．05）.（3）BacT／ALERT3D法、MGIT960法、L—J比例法完成药敏测试平均时间为10．5天、7．7天、28天，经统计存在差异显著性（P〈0．00）.结论BacT/ALERT3D法、MGIT960法、L-J比例三法对INH、SM、RFP、EMB药敏测试结果具有高的可比性。MGIT960法药敏检测时间最短。
Objective To discuss the accordance of mycobacterium tuberculosis drug sensitivity test result with equipment liquid proportion method and L-J proportion method.Methods Detecting the four anti-mycobacterium drugs （ INH, SM, RFP, EMB ） susceptibility of 107 strains mycobacterium with BacT/ALERT 3D rapid drug sensitivity test ,MGIT 960 rapid drug sensitivity test and L-J proportion method. Besults （ 1 ） The four drug （ INH, SM, RFP, EMB ） sensitivity ratio was 93%, 90%, 95%, 90% respectively with BacT/ALERT 3D rapid drug sensitivity test , MGIT 960 rapid drug sensitivity test and L-J proportion method.（ 2 ） The resistance rate of INH, SM, RFP, EMB using BacT/ALERT 3D, MGIT 960 and L-J proportion method was 27%, 28%, 24%; 12%, 13%, 19%; 25%, 25%, 22%; 7%, 6%, 9% respectively.There was no significant difference among the drug resistance rate detected by the three methods.（ 3 ）The average drug sensitivity test time using BacT/ALERT 3D, MGIT 960 and L-J proportion method was 10.5 days, 7.7 days, 28 days. There was significant difference through statistical analysis （ P 〈 0.001 ） Conclusions The four drug （ INH, SM, RFP. EMB ）sensitivity test using BacT/ALERT 3D, MGIT 960 and L-J proportion method had high comparability. The drug sensitivity test time was shortest using MGIT 960 method.
出处
《国际医药卫生导报》
2010年第9期1092-1096,共5页
International Medicine and Health Guidance News
关键词
分枝杆菌
结核
药物敏感性
方法学
Mycobacterium
Tuberculosis
drug sensitivity
Methodology
