摘要
目的研究小干扰RNA-siRNA对人甲状腺癌SW579细胞STAT3表达及细胞增殖和侵袭的抑制作用。方法将STAT3-siRNA转染给人甲状腺癌SW579细胞来沉默STAT3的表达。用逆转录聚合酶链式反应(RT-PCR)和Western blotting测定STAT3mRNA和蛋白的表达。用甲基噻唑基四唑(MTT)来分析STAT3STAT3-siRNA对癌细胞的生长抑制作用。通过RT-PCR检测MMP2和MMP9mR-NA的表达,Transwell侵袭小室实验研究癌细胞的体外侵袭能力。结果STAT3-siRNA能有效而稳定地抑制甲状腺癌SW579中STAT3的表达,下调STAT3可以在体外显著抑制肿瘤细胞的生长,在转染后24、48、72h,转染STAT3特异性的siRNA组生长抑制率显著高于对照组。siRNA沉默STAT3基因后,肿瘤细胞的体外侵袭能力明显下降。结论下调STAT3可以在体外显著抑制肿瘤细胞的生长和侵袭能力,STAT3特异性siRNA作为一种治疗肿瘤的新途径值得进一步研究。
Objective To investigate the inhibitory effect of small interfering RNA on the expression of STAT3 and proliferation and invasion in human thyroid carcinoma cell line SW579.Methods Small interfering RNA was transfected into SW579 cells to knockdown the STAT3 expression.mRNA and protein levels of STAT3 were detected by reverse transcription polymerase chain reaction （RT-PCR） and Western blot.The proliferation inhibition was determined by MTT assay.The expression of MMP2 and MMP9 mRNA were detected by RT-PCR and Transwell chambers were used to detect the invasive ability of cells in vitro.Results RNA interference efficiently and stably suppressed the STAT3 expression in STAT3 cells,and down regulation of STAT3 resulted in significantly inhibition of tumor cell growth in vit ro.At 24,48,72 h after the transfection,the growth inhibitory rates were significantly higher in specific STAT3 siRNA group than those in the control.Transwell showed that invasive ability of cells were significantly suppressed after transfection of the siRNA vector into SW579 cells.Conclusion Down regulation of STAT3 results in significant inhibition of tumor growth and suppress the invasion in vitro.The use of STAT3 specific siRNA deserves further investigation as a novel approach to cancer therapy in the future.
出处
《中国实用医药》
2010年第13期1-4,共4页
China Practical Medicine
基金
福建省自然科学基金资助项目(项目编号:2009J01146)
福建省医学创新课题资助(项目编号:2009-CXB-19)
