摘要
目的观察他莫昔芬（TAM）与全反式维甲酸（RTRA）联用对人分化型甲状腺乳头状癌细胞KAT5和滤泡状癌细胞FTC-133增殖、凋亡及侵袭能力的影响。方法将15、30μmol／LTAM，0．5、1．0μmol／LATRA分别或联合作用于KAT5和FTC-133细胞。采用噻唑蓝（MrFF）比色法检测药物作用12、24、48、72h后的细胞存活率；流式细胞术测定细胞经药物诱导48h后的早期凋亡率；Transwell侵袭实验观测药物预处理24h后的细胞侵袭能力。结果联合药物呈时间／浓度依赖性抑制KAT5和FTC-133细胞增殖，最高浓度联合组72h细胞存活率分别为18．61％和28．35％，与对照组比较差异有统计学意义（P〈0．05）；药物浓度越高，早期凋亡和侵袭抑制作用越明显，其最低浓度联合组／最高浓度联合组的凋亡率和穿膜细胞数分别为（15．46±0．51）／（24．89±0．42）、（71．33±4．31）／（47．00±3．79），（14．57±0．41）／（20．54±0．37）、（102．33±5．68）／（78．67±4．26），差异均有统计学意义（P〈0．05）。结论TAM与RTRA小剂量联合应用在抑制人分化型甲状腺癌细胞增殖、侵袭及诱导凋亡方面具有较好的协同作用。
Objective To observe the effects of tamoxifen （TAM） and all-trans-retinoic acid （ATRA） on human differentiated papillary thyroid carcinoma cell line KAT5 and follicular carcinoma cell line FTC-133 in proliferation, apoptosis and invasion in vitro. Methods Cell lines KAT5 and FTC-133 were cultured with TAM （ 15 and 30 μmol/L） and ATRA （0. 5 and 1.0 μmol/L） respectively or in combination. MTr assay was used to detect the survival rate （SR） of cells at the indicated time points （12, 24, 48 or 72 h）. The early apoptosis rate was determined by using flow cytometry （FCM） in the cell lines induced by the drug（s） for 48 h. After 24-h pretreatment withthe drug（ s）, the invasion ability of the ceils was assessed using Transwell assay. Results The proliferation of KAT5 and FFC-133 cells could be inhibited effectively by TAM and RTRA in a dose- and time-dependent manner, and SR of KAT5 and FFC-133 cells in combined maximum concentration group at 72 h was 18.61% and 28. 35% respectively （P 〈 0. 05）. With the increase of the drug concentration, the early apoptosis and inhibition of invasive ability were increased obviously. The early apoptosis rate and penetrating cells in combined minimum/maximum concentration group were （15.46 ± 0. 51）/（24. 89 ±0. 42）, （71.33 ± 4. 31）/（47.00 ± 3.79）, and （ 14. 57±0. 41）/（20. 54 ± 0. 37）, （102. 33 ± 5.68）/（78. 67 ± 4. 26） respectively, with the difference being statistically significant （P 〈 0. 05）. Conclusion Small doses of TAM combined with RTRA exert a synergic effect in differentiated thyroid cancer cells on the suppression of proliferation and invasion, and apoptosis induction. It shows a good clinical application in the treatment of thyroid carcinoma.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2012年第10期1977-1980,共4页
Chinese Journal of Experimental Surgery
基金
国家卫生部规划视听教材基金资助项目（09-VO-15）
湖北省自然科学基金资助项目（2008CDB179）
关键词
他莫西芬
维甲酸
甲状腺癌
Tamoxifen
Tretinoin
Thyroid carcinoma
