摘要
目的观察不同浓度蔗糖环境对变形链球菌葡聚糖结合蛋白c编码基因gbpc表达的影响。方法变形链球菌分别在0．5％、1．0％、5．0％蔗糖条件下培养，提取总RNA，逆转录成eDNA，利用TaqMan实时荧光定量PCR技术检测不同环境条件下变形链球菌gbpC基因的表达。结果当蔗糖浓度从0．5％升高至1．0％时变形链球菌gbpc基因表达明显上调（P〈0．05），而5．0％蔗糖较1．0％蔗糖条件下gbpc表达无明显改变（P〉0．05）；黏附较强菌株其gbpc基因表达高于黏附较弱菌株，特别是在0．5％和1．0％蔗糖环境中差异具统计学意义（P〈0．05）。结论蔗糖量从0．5％增至1．0％可促进变形链球菌gbpc基因表达，这可能是蔗糖促进变形链球菌黏附的机制之一；gbpC基因的表达量可能与变形链球菌黏附力相关。
Objective To assess the influence of concentrations of sucrose on Streptococcus mutans （S. mutans） gbpC expression. Methods Total RNA was extracted from S. mutaus after cultured in 0.5%, 1.0%, 5.0% sucrose and then reversely transcripted to cDNA. By TaqMan RT-RCR technique, we checked gbpC expression. Results Compared with 0.5% sucrose, 1.0% sucrose significantly upregulated gbpC expression（P 〈0. 05 ） ; gbpC expression of more-adherent S. mutans strains was higher than that of less-adherent strains. Conclusion The upregulation of gbpC expression by sucrose may be one of the mechanisms underlying the increased adherence; There might be some correlation between gbpC expression and S. mutaus adherence.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2010年第3期205-208,共4页
Chinese Journal of Microbiology and Immunology
基金
基金项目：广东省医学科研基金项目（A2008488）
