摘要
试验制备了更昔洛韦眼用在体凝胶剂并测定更昔洛韦的含量。采用RP-HPLC法,色谱柱:Dikma C 18柱(150 mm×4.6 mm,5μm);流动相:V(甲醇)∶V(水)=10∶90;检测波长:254 nm;柱温:室温;流速:1.0 mL/min。更昔洛韦在10.0～60.0μg/mL范围内线性关系良好(r2=0.999 5);更昔洛韦的平均回收率为99.9%,RSD为0.23%。该方法简单、快捷,回收率和重复性良好,可作为更昔洛韦在体凝胶剂质量评价的方法。
The ganciclovir in-situ ophthalmic gel was prepared and ganciclovir content in in-situ ophthalmic gel was determined by chromatography.Chromatography was performed using a Dikma C18（150 mm×4.6 mm,5 μm）column with mobile phase consisting of a mixture of methanol-water（V（methanol）∶V（water）= 10∶90）.The monitoring wavelength was set at 254 nm.The flow rate was 1.0 mL/min at room temperature.A good linearity was found in the range of 10.0～60.0 μg/mL（r2 = 0.999 5）.The average recoveries was 99.9%（RSD=0.23%）.The method is simple,rapid,satisfactory recoveries and good reproducibility.It can be used to control the quality of ganciclovir in-situ gel.
出处
《当代化工》
CAS
2010年第2期126-128,共3页
Contemporary Chemical Industry
