摘要
背景与目的真实评价免疫功能对明确肿瘤的发生发展及给予适时治疗具有重要意义。本研究旨在系统评价晚期肺癌患者免疫功能及意义。方法计数晚期肺癌患者和健康人外周血免疫细胞数;用流式细胞仪测定免疫细胞亚群比例和细胞内IL-4、INF-γ及穿孔素、颗粒酶水平;用MTT法评价淋巴细胞对肿瘤细胞株的抑制率及其增殖活性。结果晚期肺癌患者组T、B、NK细胞绝对数及亚群比例均显著低于健康组(P<0.05);但患者组的调节性T细胞(4.00±1.84)%明显高于健康组(1.27±0.78)%(P<0.05)。患者组的CD8+T细胞中IFN-γ、穿孔素及颗粒酶阳性率均显著低于健康组(P<0.05);而IL-4正好相反。患者组的免疫细胞增殖能力、PPD阳性率及对瘤细胞株的抑制率显著低于健康组(P<0.05)。结论晚期肺癌患者免疫功能较健康人明显低下。
Background and objective The actual evaluation of immunological function is significant for studing the tumor development and devising a treatment in time.The aim of this study is to evaluate the immunological function of advanced lung cancer patients systematically,and to discuss the clinical significance.Methods The nucleated cell amounts of advanced lung cancer patients and the healthy individuals were counted.The immune cell subsets and the levels of IL-4,INF-γ,perforin and granzyme in CD8＋T cells by the flow cytometry were measured.The proliferation activity and the inhibition ratio of immune cells to several tumor cell lines were evaluated by MTT assay.Results The absolute amounts and subsets of T,B,NK cells of advanced lung cancer patients were lower than the healthy individuals （P0.05）;However,the proportion of regulatory T cells of advanced lung cancer patients （4.00±1.84）% was lower than the healthy individuals （1.27±0.78）% （P0.05）.The positive rates of IFN-γ perforin,granzyme in CD8＋T cells decreased while them in IL-4 did not in the advanced lung cancer patients compared to the healthy control group （P0.05）.The proliferation activity of immune cells,the positive rate of PPD masculine and the inhibition ratio to tumor cells in the advanced lung cancer patients was lower than the healthy subsets obviously （P0.05）.Conclusion There was a significant immune depression in the advanced lung cancer patients compared to the healthy individuals.
出处
《中国肺癌杂志》
CAS
2010年第4期331-336,共6页
Chinese Journal of Lung Cancer
基金
教育部归国人员启动基金(No.2004-527)资助~~
