摘要
以刺五加种子为外植体,采用固体培养筛选出最优培养基,并成功地诱导出体细胞胚获得再生植株,以此来建立一套成熟稳定的刺五加体细胞胚再生体系。试验结果表明,最适宜的胚性愈伤组织诱导培养基为1/3MS+1.0mg/L2,4-D,其诱导率可达50%;胚性愈伤组织增殖的最适培养基为1/3MS+1.0mg/L2,4-D,其增殖倍数可达4.24倍;胚性愈伤组织在不添加任何植物生长调节物质的1/3MS培养基上培养,可促进体细胞胚的形成、萌发及植株转化率。添加10g/L蔗糖时,体细胞胚的发芽率达到90%,植株转化率达到97.8%。
Using seeds explants, an efficient plant regeneration system via somatic embryo was established for Acanthopanax senticosus . The results showed that 1/3 MS＋1.0 mg/L 2,4-D was the optimum medium for the embryogenic callus induction; 1/3 MS＋1.0 mg/L 2,4-D was the optimum medium for the embryogenic callus proliferation, propagating times was 4.24. It was increased the somatic embryo development that the embryogenic callus was transferred to 1/3 MS medium with 2,4-D lacking. Furthermore, when the medium was appended 10 g/L sucrose, the somatic embryo germination rate and plant conversion rate was 90% and 97.8% respectively.
出处
《中国农学通报》
CSCD
北大核心
2010年第10期263-266,共4页
Chinese Agricultural Science Bulletin
基金
辽宁省"十一五"攻关项目(2006215001)
"设施农业高效生产技术集成与示范"(2006215001)
辽宁省教育厅优秀人才项目(2007T159)
关键词
刺五加
胚性愈伤组织
体细胞胚
再生体系
Acanthopanax senticosus
embryogenic callus
somatic embryo
regenerate system
