摘要
目的探讨骨髓活检(BMB)中淋巴造血组织肿瘤的临床病理特征和免疫表型特点,提高诊断水平。方法 19例骨髓的淋巴造血组织肿瘤行苏木精-伊红染色法(HE)染色、过碘酸雪夫氏染色法(PAS)染色、网状纤维染色及免疫组化PV9000法系列抗体标记。结果 4例骨髓增生异常综合征(MDS)均显示造血组织增生,有小巨核细胞和原始细胞异常定位;2例急性髓系白血病(AML)有显著原始粒细胞或原始红细胞增生;2例慢性粒细胞白血病(CML)显示粒系细胞增生为主,各阶段粒细胞均可见到;3例浆细胞性骨髓瘤(PCM)和8例非霍奇金淋巴瘤(NHL)均系骨髓肿瘤性增生,两者的HE形态结合免疫组化标记能够诊断和鉴别诊断。结论 MDS诊断主要依赖病理形态学,免疫组化标记没有太大帮助;对AML、CML、PCM和NHL的诊断而言,除病理形态学观察外,各类肿瘤组织免疫组化表型分析有重要参考价值。
Objective To study the clinical and pathological features of lymphatic and hematopoietic tissue tumors in bone marrow biopsy for the improvement of diagnosis. Methods Nineteen cases of lymphatic and hematopoietic tissue tumors in bone marrow biopsy were studied by HE stain, PAS stain, reticulin stain and PV9000 antibody labeling. Results Hematopoietic tissue hyperplasia and abnormal localization of small megakaryocyte and immature precursors appeared in 4 cases of myelodysplastic syndromes （MDS）. Myeloblast or proerythroblast hyperplasia prominently appeared in 2 cases of acute myeloid leukaemia （AML）. Granulocytes hyperplasia in various stages appeared in 2 cases of chronic myelogenous leukemia （ CML）. Neoplastic hyperplasia in bone marrow occurred in 3 cases of plasma cell myeloma （ PCM） and 8 cases of Hodgking lymphoma （NHL）, both of which can be diagnosed and differentiated by HE shape together with immunohistochemical makers. Conclusions The diagnosis of MDS primarily depends on pathomorphology with little help by immunohistochemical marks. For the diagnosis of AML CML PCM and NHL, the immunophenotype of tumor tissues has very important reference value besides pathomorphological observation.
出处
《实用医院临床杂志》
2010年第3期39-42,共4页
Practical Journal of Clinical Medicine
关键词
骨髓活检
造血系统肿瘤
淋巴组织肿瘤
形态学
免疫组织化学
Bone marrow biopsy
hematopoietic tissue tumors
Lymphatic tissue tumors
Morphology
Immunohistochemistry
