摘要
单克隆抗体ＥＤ＿１、ＥＤ＿２可识别与巨噬细胞抗原提呈功能相关的决定簇。我们用免疫组织化学法及组织化学法显示铁过剩、铁缺乏对脾脏单核巨噬细胞系统ＥＤ＿１、ＥＤ＿２表达及吞噬墨汁颗粒功能的影响。发现铁过剩时红髓及白髓的巨噬细胞、树突状细胞内可见大量含铁血黄素颗粒沉积，红髓ＥＤ＿１、ＥＤ＿２表达及墨汁颗粒吞噬功能减弱，铁缺乏时ＥＤ＿１、ＥＤ＿２表达及吞噬功能增强。认为铁过剩、铁缺乏可通过改变ＥＤ＿１、ＥＤ＿２表达及吞噬功能进而影响其抗原提呈功能及动物的抗感染力。
onoclonal antibodies ED_1 and ED_2 can recognize epitopes on the spleen macrophage related withits antigen presenting activity. Immunohistochemical and histochemical methods were used to showthe influence of iron overload and iron deficiency on the expression of ED_1, ED_2 and phagocytic func-tions of the macrophages in the spleen. We found that iron overloaded rats had large amounts ofhemosiderin deposits in the macrophages of red and white pulp with reduced expression of ED_1,ED_2and phagocytic function. It is suggested that iron concentration can regulate the expression of ED_1,ED_2 and phagocytic activity of the macrophage, which may in turn influence its antigen presentationand anti-infection functions.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
1995年第1期21-24,T000,共5页
Chinese Journal of Pathology
基金
国家自然科学基金
国家教委及日本永和协会资助项目
