摘要
用荧光分光光度法研究了在模拟人体生理条件pH=7.38下,氯霉素与牛血清蛋白结合反应特征,研究发现氯霉素对牛血清蛋白有较强的荧光猝灭作用,计算得到牛血清蛋白与氯霉素在17℃和37℃下静态猝灭的猝灭常数分别为1.41×10^(12)L·mol^(-1)·s^(-1)和1.35×10^(12)L·mol^(-1)·s^(-1),结合常数分别为1.29×10~5和2.54×10~5L·mol^(-1),结合点位数均为1。根据热力学参数说明了氯霉素与牛血清蛋白相互作用以疏水作用为主,同时也存在静电引力。
The binding characteristics of chloramphenicol and bovine serum albumin were studied by fluerescenee and synchronous fluerescence spectroscopy in aqueous solution. The results showed that chloramphenieol had a powerful ability to quench the BSA fluerescence via a nonradiative energy transfer mechanism in the pH7.38 Tris - HCl buffer system. The quenching rate constants, the binding constants and the binding sites of the static quenchingg were calculated at different temperatures. And according to the themodynamie parameters calculated the binding of BSA and chloramphenicol is mainly attributed to the hydrophobic interaction, partly static force.
出处
《化工时刊》
CAS
2010年第4期52-54,共3页
Chemical Industry Times
关键词
氯霉素
牛血清蛋白
荧光猝灭
热力学常数
chloramphenicol bovine serum albumin fluerescence quenching themodynamic parameters
