摘要
目的:探讨趋化因子CCL19在人头颈鳞癌细胞活性中的作用。方法:应用噻唑蓝比色(MTT)法检测不同浓度顺铂对人头颈鳞癌原发细胞系(PCI-4A)及淋巴结转移细胞系(PCI-4B)的生长抑制作用,观察不同浓度的CCL19对顺铂作用的影响,应用透射电镜技术观察4B细胞形态学变化,流式细胞仪(FCM)检测CCL19对顺铂诱导的4B细胞周期及凋亡率的影响。使用SPSS11.0软件包进行统计学分析。结果:顺铂对4B细胞的生长抑制率高于4A细胞,CCL19可以显著降低顺铂对4B细胞的生长抑制(P<0.05),但对4A细胞无明显作用(P>0.05),CCL19对顺铂作用的4B细胞的影响是通过形态学上向正常状态转化、减少细胞在G1期的停滞、减少细胞的凋亡率而产生的。结论:顺铂在人头颈鳞癌淋巴结转移细胞(PCI-4B)中的作用强于原发细胞(PCI-4A),CCL19可以显著降低顺铂在PCI-4B细胞中的作用,提高细胞的活性,这为探讨临床头颈鳞癌的化疗耐药机制提供了一个新的思路。
PURPOSE：To study the role of CCL19 on the viability,cell-cycle and apoptosis of head and neck squamous cancer cells. METHODS：We assayed the growth arrest induced by cisplatin in 4A cells and 4B cells by MTT,detected the role of CCL19 on the effect of cisplatin and observed the change of morphology by transmission electron microscope,evaluated cell-cycle and apoptosis of 4B cell pretreated by CCL19 and cisplatin via FCM. Statistical differences between the two groups were evaluated using the unpaired Student＇s t test. All statistical analyses were performed with SPSS 11.0 software package. RESULTS：The growth arrest of 4B cell by cisplatin was significantly higher than 4A（P〈0.05）. CCL19 could protect 4B cell against inhibition,but no effect on 4A. CCL19 contributed to 4B by promoting to normal morphology,reducing G1 phase arrest,inhibiting apoptosis. CONCLUSIONS：Cisplatin works better in 4B cell than in 4A cell,and CCL19 can inhibit its effect,which provide a new way to treat head and neck squamous cancer.
出处
《上海口腔医学》
CAS
CSCD
2010年第2期158-161,共4页
Shanghai Journal of Stomatology
基金
国家自然科学基金(30672331)
辽宁省教育厅科研基金(2009A755)~~
