摘要
目的:探讨联合应用血塞通和复方丹参注射液对大鼠急性脊髓损伤的治疗作用及其机制。方法:采用改良的A llen法将48只SD大鼠制成脊髓中度损伤模型,随机分为4组,分别给予血塞通(PNS组)、复方丹参注射液(丹参组)、两药联合应用(联合组)及生理盐水(对照组)治疗,观察比较治疗后动物不同时间的运动功能评分、斜板试验和运动诱发电位,并采用原位末端标记(TUNEL)法定量观察受损伤脊髓组织的胶质细胞凋亡指数。结果:运动功能评分、斜板试验、运动诱发电位的检测和胶质细胞的凋亡指数各治疗组与对照组比较均有显著性差异(P<0.05),联合治疗组与PNS组及丹参组比较有显著性差异(P<0.05)。结论:血塞通对大鼠急性脊髓损伤有治疗作用,联合应用血塞通与复方丹参注射液对大鼠脊髓损伤具有协同治疗作用;血塞通可能通过抑制胶质细胞凋亡发挥其治疗作用。
Objective： To investigate the effect and mechanism of combined use of Xuesaitong and compound Tanshin injection on acute spinal cord injury in rats. Methods： 48 rats with moderate spinal cord injury established by a modification of Alien method, were randomly divided into 4 groups in which Xuesaitong, compound Tanshin injection, the two drugs in combination and normal saline were administered respectively. Locomotor and neurological functions were evaluated using Basso-Beattie-Bresnahan （BBB） scores, and the angle of clivus test and the motor evoked potential（MEP） were performed at 2,4,6 weeks after injury. Meanwhile, apoptotic index of glial cells was detected and analysed with in situ end labeling. Results： Significant diferences （ P 〈 0.05 ） were evidenced between the treated group and the control group and between the combined and the single therapy groups in BBB scores, the angle of clivus test, motor evoked potential and apoptotic index of glial cells. Conclusion： Xuesaitong has protective effect on acute spinal cord injury in rats ,which may be by the inhibition of glial cell apoptosis. Meanwhile ,combination of Xuesaitong and compound Tanshin injection has synergistic effect .
出处
《现代医学》
2010年第2期129-132,共4页
Modern Medical Journal
关键词
血塞通
复方丹参注射液
脊髓损伤
大鼠
Xuesaitong
compound Tanshin injection
spinal cord injury
rats
