摘要
目的调查土壤中军团菌的污染状况并探讨土壤中军团菌的污染来源。方法于2009年7—9月,在江苏省南京市冷却塔水军团菌培养法阳性的公共场所集中空调冷却塔0～2m处及距离冷却塔>2km的居民区分别采集20件和10件土壤样品。普通聚合酶链反应(PCR)方法扩增军团菌属5SrRNA基因,巢式PCR方法扩增嗜肺军团菌种Mip基因,琼脂糖凝胶电泳检测扩增产物。结果冷却塔旁土壤和居民区土壤中军团菌阳性率分别是80%(16/20)和70%(7/10);嗜肺军团菌阳性率分别是55%(11/20)和0。冷却塔旁土壤中军团菌的阳性率与居民区土壤比较,差异无统计学意义(χ2=0.373,P=0.542)。结论环境土壤中存在军团菌,受集中空调冷却塔水污染的土壤中可检出嗜肺军团菌。
Objective To investigate the contamination condition and source of LegtoneUa m soil. Metnods the samples ol soil were collected in the vicinity of cooling tower and the residential area. PCR based on 5S rRNA gene was used to detect Legionella. Nested-PCR based on Mip gene was used to detect LegioneUa pneumophila. Results The positive rate of Legionella in the soil samples in the vicinity of cooling tower and the residential area were 80%（16/20） and 70%（7/10） respectively. The positive rate of Legionella pneumophila was 55% （11/20） and 0 respectively. There was no significant difference in the positive rate of Legionella between soil in the vicinity of cooling tower and the residential area （χ2=0.373, P=0.542）. Conclusion Soil in the vicinity of cooling tower of the central air conditioning system can be contaminated by Legionellapneurnophila.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2010年第3期195-197,共3页
Journal of Environment and Health
基金
国家"十一五"科技支撑课题(2006BAI19B04)
关键词
聚合酶链反应
军团菌
嗜肺军团菌
土壤
Polymerase chain reaction
LegioneUa
LegioneUapneumophila
Soil
