摘要
目的:探讨胃肠道弥漫性大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)中之染色体t(14;18)(q32;q21)易位及Bcl-2基因扩增与DLBCL亚型分类及病人预后间的关系;探讨其在胃肠道DLBCL发病中的作用机制。方法:应用荧光原位杂交(fluorescence in situ hybridization,FISH)技术检测45例胃肠道DLBCL组织中之t(14;18)(q32;q21)染色体易位及Bcl-2基因扩增情况;应用免疫组化染色法检测该45例胃肠道DLBCL中之Bcl-2蛋白的表达。结果:在45例胃肠道DLBCL病人中,10例(22%)存在t(14;18)(q32;q21)易位,10例(22%)存在Bcl-2基因扩增。t(14;18)(q32;q21)易位阳性者与阴性者间的亚型分类比例差异有统计学意义(P<0.01)。Bcl-2基因扩增阳性者与阴性者间之生存时间差异有统计学意义(P<0.05)。而t(14;18)(q32;q21)染色体易位及Bcl-2基因扩增与Bcl-2蛋白表达间均属无关(P>0.05)。结论:t(14;18)(q32;q21)染色体易位与胃肠道DLBCL的免疫表型分类相关,生发中心B细胞(germinal center B cell-like,GCB)型与非GCB型间存在分子遗传学差异。而检测Bcl-2基因扩增对判断胃肠道DLBCL病人的预后具有重要意义。
Objective To study the correlation between Bcl-2 gene abnormalities and the subclassification of gastrointestinal diffuse large B-cell lymphoma（DLBCL）.To investigate the relationship between Bcl-2 gene abnormalities and the prognosis of patients with gastrointestinal DLBCL.Methods Forty-five cases of gastrointestinal DLBCL were enrolled.t（14;18）（q32;q21） chromosomal translocation and Bcl-2 gene amplification were detected by fluorescence in situ hybridization（FISH）.Bcl-2 protein was detected by immunohistochemical staining.Results There were 10 cases（22%） with t（14;18）（q32;q21） chromosomal translocation and 10 cases（22%） with Bcl-2 gene amplification among the 45 cases of gastrointestinal DLBCL.There existed a significant difference of subclassificaion between t（14;18）（q32;q21） chromosomal translocation positive and negative cases（P0.01）.There existed a significant difference of survival time in patients who had or not Bcl-2 gene amplification（P0.05）.Conclusions The subclassification of colorectal cancer is closely correlated with t（14;18）（q32;q21） chromosomal translocation.Detecting Bcl-2 gene amplification is helpful to predict the prognosis of gastrointestinal DLBCL.
出处
《外科理论与实践》
2010年第2期143-147,共5页
Journal of Surgery Concepts & Practice
关键词
胃肠道淋巴瘤
弥漫性大B细胞淋巴瘤
荧光原位杂交
预后
Gastrointestinal lymphoma
Diffuse large B-cell lymphoma
Fluorescence in situ hybridization
Prognosis
