摘要
目的观察肿瘤坏死因子-(TNF-)、白介素-1(IL-1)、干扰素-(IFN-)及白介素-4(IL-4)培养的人脐静脉内皮细胞(HUVEC)内皮细胞蛋白C受体(EPCR)和磷酸化P38(P-P38)的表达。方法分别采用逆转录聚合酶链反应(RT-PCR)、WESTERNBLOT技术测定正常对照组、TNF-组、IL-1组、IFN-组及IL-4组培养的EPCR mRNA、蛋白及P-P38蛋白的表达。结果TNF-组、IL-1组EPCR mRNA含量均较正常对照组低(p<0.01)。IL-1组、TNF-组EPCR蛋白含量较正常对照组低(p<0.01或p<0.05)。TNF-组、IL-1组磷酸化P38蛋白含量均较正常对照组高(p<0.01)。结论细胞因子TNF-、IL-1可以从基因、蛋白水平减少HUVEC上EPCR的表达,其调节机制可能是通过P38丝裂原活化蛋白激酶途径实现的。
Objective To investigate the expression of endothelial protein C receptor（EPCR） mRNA and phosphorP38 protein in human umbilical vein endothelial cells（HUVECs） cultured with tumor necrosis factor-（TNF-）,Interleukin-1（IL-1 ）,interferon-（IFN-） and interleukin-4（IL-4）.Methods The EPCR mRNA,protein and phosphor protein of P38 in HUVECs were detected by reverse transcription polymerase chain reaction（RT-PCR） and western bloting（WB） respectively in normal control group,TNF-group,IL-1 group,IFN-group and IL-4 group.Results The expressions of EPCR mRNA were significantly lower in TNF-group and IL-1 group than that in normal control group（all p〈0.01）;the expression of EPCR protein was significantly lower in IL-1 group than that in normal control group（p〈0.01）;the expression of EPCR protein was significantly lower in TNF-group than that in normal control group（p〈0.05）;the expressions of phosphor-P38 protein were significantly higher in TNF-group amd IL-1 group than that in normal control group（all p〈0.01）.Conclusion TNF-and IL-1 can decrease EPCR mRNA and protein expression of HUVECs,which can be achieved through increasing the expression of phosphor-P38.
出处
《现代实用医学》
2010年第3期256-258,共3页
Modern Practical Medicine
