摘要
探讨了大豆脂肪酸快速气相色谱分析方法,不需从原料中提取脂质,直接将磨碎原料中的脂质皂化、甲脂化后,利用色谱柱HP519091 J-413,进行气相色谱分析。结果表明:软脂酸、硬脂酸、油酸、亚油酸和亚麻酸的出峰时间依次为6.57、7.83、8.73、8.86、9.06 min。这种方法的优点是快速、操作方便、节省药品;即使在较低的杂交世代样品很少,用普通法不易提取脂质时,此法也能顺利地完成分析工作。
This paper discussed the rapid determination on fatty acids content by gas chromatography in soybean, which justsa- ponificate and Methyl esterificate the lipids from grinding soybean seeds directly without having to extract lipids from soybean, then get the gas chromatography determination by chromatography column HP519091J-413. The time of peak value of palmitic acid, stearic acid, oleic acid, linoleic acid and linolenic acid was6.57, 7.83, 8.73, 8.86, 9.06 rain, respectively. The method proved to be time and material saving, and easy to operate. The method also helpful for soybean acids determination in early generation for it only needs few samples.
出处
《大豆科学》
CAS
CSCD
北大核心
2010年第2期358-360,共3页
Soybean Science
基金
黑龙江省教育厅资助项目(115350033)
关键词
大豆
脂肪酸
气相色谱分析
Soybean
Fatty acids
Gas chromatography
