摘要
目的探讨神经干细胞（NSCs）移植入脑出血模型大鼠脑内后对脑组织内IL-6、TNF-α表达的影响。方法体外培养大鼠NSCs；选取SD大鼠180只，随机取60只大鼠为健康对照组（A组），余120只采用Ⅳ型胶原酶诱导法建立脑出血模型，并随机分成两组：B组60只为单纯脑出血组；C组60只为脑出血＋NSCs移植组，三组大鼠分别在NSCs移植后6h、1d、3d、7d、14d进行肢体运动功能评分后，处死取脑组织匀浆，以ELISA方法分别检测三组大鼠脑组织内5个时间点IL-6、TNF-α的表达，并用统计学的方法分析。结果与单纯脑出血组比较，脑出血＋NSCs移植组能够显著降低IL-6、TNF-α表达（P〈0．05）和促进肢体功能恢复（P〈0．05）。结论大鼠NSCs脑内移植能有效降低脑出血模型大鼠脑内IL-6、TNF—α的表达，并能够改善运动神经功能缺损。
Objective To investigate the effect of neural stem cells from embryonic brain of rat transplanted into the intracerebral Hemorrhage （ICH） model of rat brain on the expression of inflammatory factors （ IL - 6,TNF - α）. Method Neural stem cells, enough to be transplanted, were cultured in vitro. 120 intracerebral hemorrhage rat models were divided into group B （60 rats） for pure cerebral hemorrhage group and group C （60 rats） for cerebral hemorrhage ＋ NSCs transplantated group. Another 60 rats were randomly selected as group A for normal control group. Behavior testing for each group was conducted at 6 h, 1 d,3d ,7 d,14 d after ICH respectively using limb function tests. Then rat models were killed. The brain tissue of rats were homogenated and frozen at - 70℃. The expression of IL - 6 and TNF - α in brain tissues of three groups were detected at five time points described above respectively. The data were dealed by statistical methods. Results The expression of inflammatory cytokines （ IL - 6 and TNF - α） of group C was significantly lowered than that of group B （ P 〈 0. 05 ）. The promotion of restoration of limb function of group C was more significant than that of group B. Conclusions Neural stem ceils transplanted into brain of rat models could effectively reduce the expression of inflammatory cytokines （ IL - 6 and TNF - α） in brain of ICH rat model and improve motor nerve function deficits.
出处
《中华神经外科杂志》
CSCD
北大核心
2010年第3期272-276,共5页
Chinese Journal of Neurosurgery
关键词
神经干细胞
脑出血
移植
炎性反应因子
Neural stem cells
Cerebral hemorrhage
Transplantation
Inflammatory factors
