摘要
目的研究细胞周期相关因子sonic hedgehog（SHH）在大鼠自体移植静脉中的表达变化及其与内膜增生的关系。方法雄性Wistar大鼠24只，8周龄，体质量140g。建立颈静脉一腹主动脉移植模型，分别于术后14d、28d各处死12只大鼠，取材移植血管，免疫组化检测SHH蛋白在移植血管新生内膜中的表达，Western blot法观察SHH及细胞增殖核抗原（PCNA）的表达，Real—timePCR定量检测SHHmRNA的表达；以对侧颈静脉作为对照。结果免疫组化结果显示，正常静脉、术后14d和术后28d移植静脉SHH＋细胞数比例分别为（2．0±0．5）％、（39．4±0．4）％和（63．0±0．3）％，正常静脉与移植静脉差异有统计学意义（P〈0．01）。Western blot法显示术后SHH表达水平增加并与PCNA表达呈正相关（r=0．808，P〈0．01）。Real—time PCR结果显示术后14d组和术后28d组的SHH mRNA表达增加，分别为对照组含量的9．5和23．8倍。结论自体静脉移植术后，SHH在新生内膜中高表达并可能与细胞增殖相关。
Objective To study the expression of cell cycle related factor sonic hedgehog （SHH） in autogenous vein graft and its relation with neointima formation. Methods Autogenous vein graft model were established in 24 male Wistar rats of 8 weeks old and 140 g weight, by transplanting the left jugular vein to intra renal abdominal aorta with microsurgical technique. Graft veins were harvest at 14 d and 28 d after transplantation. The immunohistoehemistry and Western blot were used to detect the SHH and PCNA expression in the vein graft. At the same time SHH mRNA was measured by quatitative Real-time PCR. The opposite normal veins served as control. Results Histolaogical staining showed that the percent of SHH ＋ cells was only （2. 0 ± 0. 5 ） % in the normal vein, but was much more in the vein graft after surgery, as （39. 4 ± 0. 4） % and （63.0 ± 0. 3 ） % respectively （ P 〈 0. 01 ）. The expression of SHH and PCNA were both elevated in the vein graft. There was a positive correlation between them which indicated by Western blot （ r = 0. 808, P 〈 0. 01 ）. The SHH mRNA content also increased in vein graft to 9. 5 and 23.8 folds of that in control. Conclusion SHH is upregulated in autogenous vein grafts and may correlated with the proliferation of vascular smooth muscle cells.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2010年第7期539-542,共4页
Chinese Journal of Surgery
基金
国家自然科学基金资助项目（30370551）
