摘要
目的观察在动脉粥样硬化（AS）形成过程中，运动对实验小鼠主动脉细胞间黏附分子-1（ICAM-1）及血管细胞黏附分子-1（VCAM-1）表达的影响。方法选取8周龄ApoE基因敲除小鼠饲以“西方饮食”饲料建立AS模型，将其分为ApoE-安静组及ApoE^-/-运动组，ApoE^-/-运动组给予持续12周中等强度跑台运动；另同时选取c57BL／6J小鼠作为空白对照组（C57安静组），给予普通生长繁殖饲料喂养。于实验进行12周时采用免疫组化法检测各组小鼠主动脉ICAM-1及VCAM-1表达情况。结果C57安静组主动脉无ICAM-1、VCAM-1阳性表达，ApoE^-/-安静组小鼠主动脉可见大量ICAM-1、VCAM-1阳性表达，在AS早期阶段，以内皮层阳性表达较明显；在纤维斑块及粥样斑块阶段，阳性染色则主要分布于斑块深部脂质核心区，内皮层呈低水平表达，ICAM-1、VCAM-1阳性表达面积相近；运动可促使ApoE^-/-小鼠ICAM-1、VCAM-1阳性表达面积明显减少（P〈0．05）。结论运动能下调AS小鼠主动脉ICAM-1、VCAM-1表达，这可能是运动抗AS的重要机制之一。
Objective To investigate the effects of exercise on the expression of intercellular adhesion molecule-1 （ICAM-1） and vascular cell adhesion molecule-1 （VCAM-1） in the aorta. Methods Eight-week-old apolipoprotein E （ApoE） knockout mice （ApoE^-/- ） were fed a ＂ western diet＂ for 12 weeks to establish an atherosclerotic model, and C57 mice were used as a homologous group. They were divided into three groups： a C57 sedentary group （CC group, n = 15） , an ApoE ^-/- sedentary group （AC group, n = 15） and an ApoE^-/- exercise group （AE group, n = 15）. Mice in the exercise group ran on a treadmill at 10 m/min for 30 min progressing up to 13 m/min for 60 rain gradually for 5 d/week for a total 12 weeks. At the end of experiments, immunohistochemical analysis revealed the expression of ICAM-1 and VCAM-1 in the aortas of the mice. Results Immunostaining revealed no expression of ICAM-1 or VCAM-1 in the mice of the CC group. However the immunostaining revealed ICAM-1 and VCAM-1 in the endothelia, lesions and media in the ApoE^-/- mice in the AC and AE groups. The expression was less intense in fibrous plaque and atheromatous plaque of the endothclium, but was pronounced at an early stage. Meanwhile ICAM-1 and VCAM-1 expression were detected in regions of the endothelium without lesions. It was noticed that exercise significantly decreased the relative area showing ICAM-1 or VCAM-1 expression. Conclusions Moderate intensity exercise for 12 weeks can decrease the expression of ICAM-1 and VCAM-1 in ApoE^-/- mice. This may he one of the anti-atherogenie mechanisms of exercise.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2010年第2期81-85,共5页
Chinese Journal of Physical Medicine and Rehabilitation
基金
上海市教育委员会科研创新项目（09YZ152）,上海师范大学科研资助项目（SK200752）
