摘要
目的建立布比卡因在硬膜外麻醉犬体内死后再分布动物模型,观察温度对其死后再分布规律的影响。方法雄性杂种犬18只,盐酸布比卡因(5mg/kg)硬膜外麻醉致死,随机分3组,分别置于室温(20～23℃)、4℃和-20℃,于死后0、2、4、8、24、48、72、96、120h同一尸体提取心血、周围血、肝和大脑,GC-NPD、GC-MS法检测其中布比卡因含量,比较其各组含量变化。结果室温时心血、周围血和大脑的死后再分布现象较明显且复杂,4℃时死后再分布现象较室温下弱且缓慢,-20℃时4种检材中布比卡因的再分布现象进一步减弱。结论布比卡因在硬膜外麻醉致死犬体内可发生死后再分布,低温保存可延缓或阻止其发生。
Objective To establish the models of postmortem redistribution （PMR） in dogs with epidural anesthesia and to investigate the effect of temperature on the PMR of Bupivacaine. Methods Eighteen male dogs were executed by epidural anesthesia with a dose of 5 mg/kg bupivacaine hydrochloride and randomly divided into three groups, room temperature（20-23 ℃） group, 4℃ group and -20℃ group. The cardiac blood, peripheral blood, liver and cerebrum were collected at 0, 2, 4, 8, 24, 48, 72, 96, 120h postmortem. The contents of bupivacaine in those samples were analyzed by GC-NPD and GC-MS, the difference among three groups were compared. Results The bupivacaine PMR of room temperature group was evident and complex in cardiac blood, peripheral blood and cerebrum. The PMR of 4℃ group was weaker and slower than that of normal temperature group. The bupivacaine PMR of the -20 ℃ group was the weakest in three groups. Conclusion PMR of bupivacaine will happen in epidural anesthesia death dogs, but it could be delayed or prevent by low temperature storage.
出处
《法医学杂志》
CAS
CSCD
2010年第2期112-115,共4页
Journal of Forensic Medicine
基金
山西省高等学校中青年拔尖创新人才支持计划项目(TYMIT)
"十一五"国家科技支撑计划项目(2007BAK26B05)
关键词
法医毒理学
布比卡因
温度
麻醉
硬膜外
犬科
死后再分布
forensic toxicology
hupivacaine
temperature
anesthesia, epidural
canidae
postmortem redistribution
