摘要
以Nested－PCR方法从人肝cDNA基因文库中扩增出编码人血小板生成素（hTPO）前153个氨基酸的氨基端功能区cDNA；在扩增中，采用非连续多核甘酸定点突变的方法．将翻译起始的七个氨基酸的原核中不常用的密码子同又突变成使用频率较高的密码子，以便于其在大肠杆菌中表达。序列测定证实了预期的结果。
The cDNA. coding human thromhopoietin (hTPO ) amino - terminal functional region 153 amino acids, was obtained by Nested -PCR from human liver cDNA library. Using the synthetic primers, seven genetic codes was replaced by the synonmous codes used more frequently in E, coli during Nested -PCR, The sequencing confirmed the expected result.
出处
《生物技术》
CAS
CSCD
1998年第5期10-12,共3页
Biotechnology
关键词
人血小板生成素
cNDA
定点突变
序列测定
human thrombopoietin (hTPO), Nested-PCR, cDNA site-directed mutagenesis, DNA sequencing
