摘要
目的建立用高效液相色谱(HPLC)测定独角莲膏中连翘苷的含量的方法。方法采用HypersilODS2(5.0×200mm)色谱柱,乙腈与水(24:76)的混合液为流动相;流速为1.0 ml/min,柱温为30℃,检测波长227nm。结果连翘苷进样量在0.1424μg^0.712μg时线性良好(r=0.99998),平均回收率为99.22%,RSD为2.16%。结论该方法准确、简便、重现性好、专属性强,可用于该产品中连翘苷含量的测定。
Objective To establish a method to determine the content of phillyrin of Dujiaoliangao by HPLC. Methods The column used in the analysis was Hypersil ODS2（5.0×200 ram）with the temperature of 30℃ and detected at 227nm, with the mobile phase of acetonitrile and water（24： 76）. The flow rate of the mobile phase was 1.0ml/min. Results The linear range of phillyrin was 0. 1424μg-0. 712μg（r= 0. 99998）. The average recovery rate was 99.22~/6 with a RSD of 2. 16%. Conclusion The method is accurate and simple with a good reproducibility, and can be used to determine the content of phillyrin of Dujiaoliangao.
出处
《四川解剖学杂志》
2010年第1期6-7,共2页
Sichuan Journal of Anatomy
基金
贵阳医学院青年教师基金资助项目(№:K2007-61
67)
关键词
植物制剂
色谱法
高效液相
连翘苷
独角莲膏
plant preparations
chromatography
high pressure liquid
Phillyrin
Dujiaoliangao
