摘要
目的探讨百草枯中毒致急性肺损伤中热休克蛋白70(HSP70)的表达。方法72只健康Wistar大鼠随机平均分成3组,分别腹腔注射生理盐水(A组)、20mg/kg百草枯(B组)和40mg/kg百草枯(C组)。分别于1,2,3,6,12和24h留取标本,应用免疫组织化学染色、Western blot方法测定肺组织中HSP70的蛋白表达。结果B组和C组大鼠肺组织发生急性肺损伤。B组大鼠2h后HSP70表达与A组相比开始增强,6h为表达高峰,12h表达减少,24h表达水平与A组相当。C组1h后HSP70表达与A组相比明显增强,3h达到高峰,24h后与A组水平一致。结论百草枯诱导的急性肺损伤可引起支气管、细支气管和肺泡上皮细胞HSP70应激性表达,提示HSP70对肺损伤起保护作用。
Objective To investigate the expression of heat shock protein-70（HSP70）in acute lung injury induced by paraquat poisoning. Methods A total of 72 Wistar rats were randomly divided into 3 groups （n =24 each group）and intraperitoneally injected with normal saline in group A,20 mg/kg paraquat in group B,and 40 mg/kg paraquat in group C. The lungs of rats were excised 1,3,6,12,and 24 hours after the injection,respectively. The expression of HSP70 in the lung tissue was detected by immunohistochemistry and Western blot. Results Paraquat induced acute lung injury in groups B and C. In group B,the expression of HSP70 increased at 2 hours,reached the peak at 6 hours,decreased at 12 hours,and returned to the baseline level at 24 hours. In group C,the expression of HSP70 increased at 1 hour,reached the peak at 3 hours,and returned to the baseline level at 24 hours. Conclusion Paraquat-induced acute lung injury may contribute to the enhanced expression of HSP70 in the bronchus,bronchiole,and alveolar epithelial cells,which indicates that HSP70 has a protective effect on lung injury.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2010年第3期212-214,227,共4页
Journal of China Medical University
