摘要
以小诺霉素（Micromomicin,MCR)产生菌棘孢小单胞菌（Micromonosporaechinospora）为出发菌株，诱变筛选得到一株链霉素抗性菌株，抗性菌株的原生质体分别用紫外线照射和热处理致死，通过单亲致死原生质体融合，以链霉素为选择条件选出融合株，经摇瓶发酵并结合薄层层析扫描（ThinLayerCharamotography，TLC）分析，筛得4株MCR百分含量高于亲株的融合子，MCR百分含量达到90％，效价1076u／ml，分别比亲株提高15％和11％。
Micromonosp ora echinospora. a micronomicin producer, was mutated then screened a streptomicin resistance strain. The fusion bodies were screened with the pro toplast of single parent strain inactivation by U. V and heat. respectively. We obtained 4 strains of higher MCR percentage than their parent strains by fermentation and Thin -Layer Charomatography analysis. The MCR percentage and titer were higher than their parent strains,15% and 11% respectively.
出处
《生物技术》
CAS
CSCD
1998年第6期6-8,13,共4页
Biotechnology
关键词
棘孢小单胞菌
小诺霉素
原生质体融合
电场诱导
Micromonospora echinospora, Micromomicin MCR, Thin-Layer Charomatography
