摘要
目的:检测卵巢上皮性癌组织中磷酸化信号转导及转录活化因子3(p-STAT3)和Ki67蛋白的表达。方法:应用免疫组织化学SP法检测35例卵巢上皮性癌组织(高分化11例,中分化12例,低分化12例;Ⅰ、Ⅱ期16例,Ⅲ、Ⅳ期19例;有淋巴结转移20例,无淋巴结转移15例)、20例良性卵巢上皮性肿瘤组织及30例正常卵巢组织中p-STAT3和Ki67蛋白的表达。结果:正常卵巢组织、良性卵巢上皮性肿瘤组织及卵巢上皮性癌组织中p-STAT3的表达水平分别为(43.20±3.18),(50.12±4.36)及(92.57±5.26),3者相比,差异有统计学意义(F=7.605,P=0.001);Ki67的表达水平分别为(7.69±1.21)、(12.53±2.62)及(16.18±5.32),3者相比,差异有统计学意义(F=3.925,P=0.029)。卵巢上皮性癌组织中p-STAT3和Ki67的表达与临床分期、分化程度及淋巴结转移有关(p-STAT3:t/F=6.921、3.865和7.763,P均<0.05;Ki67:t/F=6.917、3.870和4.021,P均<0.05)。卵巢上皮性癌组织中p-STAT3与Ki67的表达呈正相关(rS=0.563,P<0.001)。结论:卵巢上皮性癌组织中p-STAT3与Ki67的表达升高,联合检测2者对判断卵巢上皮性癌的恶性程度和预后有一定价值。
Aim ： To detect the expressions of p-signal transducers and activators of transcription3 （STAT3） and Ki67 in epithelial ovarian carcinoma tissue. Methods ：The expressions of p-STAT3 and Ki67 in normal ovary （ n = 30） , benign epithelial ovarian tumor（ n = 20） , and epithelial ovarian carcinoma tissue（ n = 35 ; 11 cases of well differentiation, 12 cases of moderate differentiation,and 12 cases of poor differentiation;16 cases of stage I and 1] ,19 cases of stage Ili and IV ;20 cases with lymph node metastasis, 15 cases without lymph node metastasis） were detected by immunohistochemistry. Resuits ： There was significant difference in the expressions of p-STAT3 [ （43.20 ± 3.18 ）, （50.12 ± 4.36） , and （ 92.57 ± 5.36）] and Ki67[（7.69 ±1.21）,（12.53 ±2.62）,and （16. 18 ±5.32）] among the three kinds of tissue（F=7.605 and 3. 925 ,P 〈 0.05 ）. The expressions of p-STAT3 and Ki6？ in epithelial ovarian carcinoma tissue were related with clini- cal stage ,differentiation grade, and lymph node metastasis （ p-STAT3 ： t/F = 6. 921,3. 865 and 7. 763, P 〈 0.05 ; Ki67 ： t/F =6. 917,3. 870 and 4. 021 ,P 〈0.05）. The expressions of p-STAT3 and Ki67 in epithelial ovarian carcinoma tissue were positively correlated （rs = 0. 563, P 〈 0. 001 ）. Conclusion： The expressions of p-STAT3 and Ki67 increase in epi- thelial ovarian carcinoma tissue. Combined detection of the 2 parameters is of value in predicting the malignant degree and prognosis of epithelial ovarian carcinoma.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2010年第2期246-249,共4页
Journal of Zhengzhou University(Medical Sciences)
