摘要
目的：建立一种阿达帕林脂质体中药物含量的测定方法。方法：采用一阶导数紫外分光光度法,测定波长279 nm处的一阶导数峰-零谷值与阿达帕林脂质体中药物含量的线性关系。结果：在279 nm处一阶导数峰-零谷值可有效消除辅料磷脂对阿达帕林测定的干扰,阿达帕林在6.12~36.72μg.mL-1范围内,浓度与零谷值之间存在良好的线性关系,r=0.999 7;平均回收率为99.42%,RSD为0.72%（n=9）;日内及日间精密度的RSD均小于1%（n=5）。经配对t检验,该方法的测定结果与用HPLC法测定结果没有显著性差异（P〉0.05）。结论：该方法简便、快速、结果准确,适于测定阿达帕林脂质体中药物含量。
Objective：To establish a method for determining the content of adapalene in liposomes.Methods：The first derivative UV spectrophotometry was used to determine the content of adapalene in liposome with wavelength at 279 nm.Results：The excipients in the liposome did not interfere with the assay.Linear relationship between the values of first derivative and the concentration of adapalene was obtained in the range of 6.12~36.72μg·mL-1（r=0.9997）.The average recovery of samples was 99.42% with RSD 0.72%（n=9）;RSD of the intra-and inter-day precision were lower than 1%（n=5）.The results of first derivative spectrophotometry were not significantly different from those of HPLC method as analyzed by Paired-Samples T test.Conclusion：The method is simple,quick and accurate.It is suitable for determination of adapalene content in the liposomes.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2010年第6期534-536,共3页
Chinese Journal of New Drugs
关键词
阿达帕林脂质体
一阶导数分光光度法
含量测定
adapalene liposomes
first derivative spectrophotometry
content determination
