摘要
目的观察慢性砷中毒对小鼠海马齿状回超微结构的改变及小鼠学习记忆的影响。方法选取健康成年昆明小鼠80只,雌雄各半,分为对照组、慢性砷中毒低、中、高剂量组,每组20只,各染砷组分别以蒸馏水、1/5LD50、1/10LD50、1/40LD50As2O3灌胃,连续3个月,根据其体重变化随时调整用药剂量。灌胃结束后,采用Y-电迷宫检测各组小鼠学习记忆行为,透射电镜观察齿状回细胞超微结构的变化。结果高剂量砷中毒组学习、记忆的Y-迷宫测试次数明显高于对照组(P<0.05),电镜下观察中毒组齿状回神经元,可见部分神经细胞膜皱缩,胞体减小,核染色质电子密度增高。结论慢性砷中毒明显损害成年小鼠学习记忆的能力,可能与海马齿状回神经元受损有关。
Objective To study the effects of chronic arsenic poisoning on the change of the dentate gyrus ultrastructure and learning and memory in mice.Methods 80 healthy adult Kunming mice,weighting 20-22 g,were divided into four groups：the normal control group,the low dose group,the middle dose group and the high dose group（10 males and 10 females in each group）,which were fed with distilled water,1/5 LD50,1/10 LD50 and 1/40 LD50 AS2O3 for 3 months respectively,and the dose was adjusted according to their weight changes.Then the ability of learning and memory was tested by a Y-maze,and the ultra-structure of the dentate gyrus was observed by transmission electron microscopy.Results The ability of learning and memory of the high dose group was significantly lower than that of the normal control group（P〈0.05）,and some neurons of the dentate gyrus of each poisoned group showed a shrunken cellular membrane,diminished cell body and increased electron density of nuclear chromatin under transmission electron microscopy.Conclusion Chronic arsenic poisoning might damage the ability of learning and memory in mice,which may be related with damaged neurons of the dentate gyrus.
出处
《山东大学学报（医学版）》
CAS
北大核心
2010年第3期34-38,共5页
Journal of Shandong University:Health Sciences
基金
[2007]筑科计合同字第6-13号
关键词
砷中毒
迷宫学习
记忆
齿状回
小鼠
Arsenic poisoning
Maze learning
Memory
Dentate gyrus
Mice
