摘要
目的:建立年轻成年大鼠下颌前伸动物模型,以X型胶原为检测指标,观察髁突软骨内成骨及其与下颌前伸持续时间的关系。方法:9周龄雌性大鼠75只,分为3组,其中2组为实验组,另一组为对照组,实验组动物戴用统一规格自制的咬合前导矫治器,分别戴用12h和24h。3组动物分别在实验后3、7、14、21、30d处死后取双侧髁突组织,使用免疫组化和原位杂交方法从蛋白水平和基因水平检测髁突软骨组织切片中X型胶原的表达情况。结果:免疫组化结果显示,24h实验组X型胶原表达量在21d时最高,且高于其他实验组;而12h实验组峰值出现在30d。原位杂交结果与免疫组化结果基本吻合。结论:功能矫治下颌前伸均能诱导年轻成年大鼠髁突软骨发生改建,但与12h间歇性加力方式相比,24h持续加力方式作用效果明显,且见效快。
Objective：To examine the relation between the alteration of condylar endochondral in response to mandibular protrusion and the action time length by establishing a model of mandibular protrusion on young adult rats. Methods： 75 female Sprague-Dawley rats aged 9 weeks were divided into two experimental groups and one control group. In two experimental groups, bite jumping appliances created forward positioning of the condyle for twelve hours and whole day, respectively. The experimental rats, together with the control rats, were sacrificed on days 3, 7, 14, 21 and 30, respectively. Tissue sections were cut in the sagittal plane through the mandibular condyle and were processed for in situ hybridization and immunostaining of type X collagen. Results： The peak of type X collagen protein expression in 24-hour experimental groups appeared on day 21 which was on the top of all experimental groups, while in 12-hour experimental groups it was found on day 30. The results of in situ hybridization were basically in agreement with the results of immunostaining. Conclusion： Both intermittent and continuous mandibular advancement can provoke endoehondral ossification in young adult rat condylar, while 24-hour ones can produce more obvious and quicker effect.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2010年第2期181-184,共4页
Journal of Practical Stomatology
关键词
安氏Ⅱ类错[牙合]畸形
下颌前伸
髁突软骨
功能矫治器
X型胶原
Class H malocclusion
Mandibular advancement
Condylar cartilage
Functional orthopedics appli- ance
Type X collagen
