摘要
本实验通过功能矫治器引导大鼠下颌前伸,采用免疫组织化学的ABC法在原位上确定髁突软骨胰岛素(insulin,Ins.)的分布,探讨其在矫形治疗中的作用及与髁突生长改建之间的关系。结果显示,髁突软骨Ins.分布呈明显的染色梯度,即生发层染色最深,阳性率较高,其次为过渡层,再次是成熟层;实验组髁突中后份,特别是生发层明显增厚,阳性细胞染色深,比例大,其余各层染色亦略深于对照组。本研究表明,髁突软骨中Ins.的分布有一定规律。髁突软骨增生旺盛时,Ins.分布较多,分化活跃时分布则减少。功能矫形治疗可使髁突软骨明显增生,Ins.分布亦随之增加,说明Ins.与髁突软骨增生密切相关。
Thepurposeofthisstudyistoevaluateinsulinchangeincondylarcartilageduringfunctionaladvancementofratmandiblebyimmunohistochemicaltechnique(ABCmethod)inordertoprovidefurtherinformationabouttheroleofinsulininorthopedicsandtherelationshipbetweenin-sulinandproliferationofcondylarcartilage.Staininggradientofinsulinisobservedincondylarcarti-lage,withthestaindeepestinthegeriminallayer,medialinthetransitional,leastinthematura-tionallayer.Theposteriorregionofcondylarcartilageintheexperimentalgroupsisshowntohavethickenedsignificantlyascomparedwiththecontrolgrups,especialythegerminallayer.Stained-positivecelsexhibitdeeperstainandhigherpercentage.Thestainintheotherlayersisalsomoreobviousthanthatinthecontrolgroups.Theresultssuggest:Insulinlevelincondylechangeswiththediferentcartilagelayers.Insulindistributionappearshigherwhencondylarchondrocytesprolif-eraterapidlyandlowerwhentheydiferentiateactively.Functionalorthopedicsmaystimulatethegrowthofcondylarcartilageandraisetheinsulinlevel.Thedemonstratesthatinsulincorrelateswithhyperplasiaofconylarchondrocytes.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
1996年第2期91-94,共4页
Chinese Journal of Stomatology
基金
高等院校博士点专项基金