摘要
目的探讨不同交变应力作用下,大鼠翼外肌成肌细胞增殖活性的变化,为交变应力下翼外肌组织的改建提供必要的基础实验数据。方法在自行研制的脉动式细胞力学系统基础上,将大鼠翼外肌成肌细胞在膜交变应力培养小室内进行培养,分别施加2.5、5.0、10.0kPa交变力学刺激于不同频率组,3H-TDR检测成肌细胞增殖活性,并将结果进行统计学处理分析。结果高频组不同大小的交变应力都有促成肌细胞增殖的趋势,与空白组比较具有明显的促增殖作用(P<0.05);在交变应力作用12h后,不同频率组间比较的规律与6h后基本一致,但是较6h具有更大的增殖活性变化。低频组组间的增殖活性变化规律基本与高频组一致,但低频组有更大的促增殖效应。结论低频率比高频率具有较高的促成肌细胞增殖活性;随着交变应力作用时间的延长,成肌细胞增殖活性在较低应变范围内(≤5.0kPa)随应变的加大而增加,较大应变具有较小的成肌细胞增殖活性增加趋势。
Objective To investigate the effects of time varied stress on the proliferation of myoblast in rats and provide the basic experimental data for the remodeling of tissue in functional orthopaedics. Methods Based on the pulsatile mechanical system founded, this study loaded different strain （2.5, 5.0, 10.0 kPa） to the myoblast of lateral pterygoid muscle. The proliferation of myoblast was detected by ^3H-TDR. Results After 6 hours under time varied strain, the significant proliferation of myoblast （P〈0.05） was observed, and the 5.0 kPa group expressed the best proliferation. After 12 hours under time varied strain, all groups expressed a better proliferation. Meanwhile, the lower frequency （0.40 Hz） had the bigger effect on the proliferation more than in the higher frequency （1.25 Hz） under the same time varied strain. Conclusion The frequency of time varied strain had also the important influence on the proliferation, the lower frequency （0.40 Hz） had the bigger effect on the proliferation more than in the higher frequency （1.25 Hz） under the same time varied strain. In the certain period of time and certain magnitude of time varied strain, the proliferation of myoblasts rised.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2007年第3期289-291,共3页
West China Journal of Stomatology
基金
国家自然科学基金资助项目(10472138和10202016)
关键词
交变应力
成肌细胞
增殖活性
time varied stress
myoblast
proliferation
