摘要
目的探讨细胞间黏附分子１（ＩＣＡＭ１）的表达及其在实验性肝纤维化发生中的作用。方法分别建立高脂饮食、低脂酒精饮食、高脂酒精饮食、四氯化碳大鼠实验性肝纤维化模型，用免疫组织化学方法观察造模肝组织中ＩＣＡＭ１的表达。结果ＩＣＡＭ１表达于肝细胞膜上，其阳性细胞多分布于汇管区周围、肝小叶内炎性细胞浸润区和坏死灶内，其阳性强度及分布与肝脏炎症、肝纤维化程度和分布相一致；在不同模型组中，ＩＣＡＭ１表达强度依高脂饮食、低脂酒精饮食、高脂酒精饮食、四氯化碳次序而增强，且以四氯化碳组最为明显（Ｐ＜００５），但前３组间差异无显著性（Ｐ＞００５）。结论在实验性肝纤维化组织中，ＩＣＡＭ１表达增加可能与肝脏炎性损伤和肝纤维化的发生有关。
Objective To explore the role of intercellular adhesion molecule 1 (ICAM 1) in experimental liver fibrosis. Methods Experimental liver fibrosis models of rats were induced by high fat diet, low fat diet plus ethanol, high fat diet plus ethanol, and carbon tetrachloride subcutaneous injection respectively. At sacrifice, liver pathologic changes were studied and ICAM 1 expression was evaluated with immunohistochemistry by using a monoclonal antibody. Results ICAM 1 expression was observed in the hepatocellular membrane; ICAM 1 positive hepatocytes were localized in zones of portal tract with focal necrosis and inflammatory damage. Staining for ICAM 1 was associated with the degree of liver inflammation and fibrosis. In the different models, the intensity of ICAM 1 expression was different; It ranged in an increasing order from high fat diet, low fat diet plus ethanol, high fat diet plus ethanol, and carbon tetrachloride model. The strongest reactivity for ICAM 1 was seen in carbon tetrachloride model ( P <0.05), the difference was significant as compared with the other models. However, there was no significant difference among the three other models ( P >0.05). Conclusion Upregulation of ICAM 1 expression in experimental liver fibrosis may be related with liver fibrogenesis.
出处
《中华内科杂志》
CAS
CSCD
北大核心
1999年第1期37-39,共3页
Chinese Journal of Internal Medicine
关键词
细胞间粘附分子
免疫组织化学
肝纤维化
Intercellular adhesion molecule 1 Liver cirrhosis Immunohistochemistry
