摘要
为寻找诊断肝硬化失代偿期并发自发性腹膜炎、胸膜炎和败血症时检测病原菌的方法。采用聚合酶链反应（ＰＣＲ）扩增真细菌域的１６ＳｒＲＮＡ基因，与Ｇｒａｍ阴性和Ｇｒａｍ阳性特异探针杂交检测肝硬化失代偿期患者胸腹水和血液中的致病菌。结果：ＰＣＲ腹水检测病原菌的阳性率９１４％；Ｇｒａｍ阴性探针斑点杂交（Ｄｏｔｂｌｏｔ）阳性率１００％；Ｇｒａｍ阳性杂交阳性率２１７％，高于细菌培养和鲎试验。结果提示：用ＰＣＲＤｏｔＢｌｏｔ杂交法检测病原菌的１６ＳｒＲＮＡ基因是诊断肝硬化自发性腹膜炎。
To find the mothed for detecting pathogens when spontaneous peritonitis, pleuritis and septicemia occur. Polymerase chain reaction targeted conserved sequence of eubacterial 16S rRNA gene, combined with dot blot hybridization was used. The results showed that positive rate of detected pathogens in ascites was 91.4%,100% and 21.7% by PCR, byhybridizating with Gram-probe and with Gram+ probe, respectively, and the positive rate of PCR Dot Blot was high than culture of bacteria. It is suggested that detection of 16S rRNA gene in ascites, pleural fluid and blood may be a good mothd to diagnose spontaneous peritonitis, pleuritis, and septicemia.
出处
《河南医科大学学报》
1998年第4期42-45,共4页
Journal of Henan Medical University
基金
河南省科技攻关资助
关键词
肝硬化
16S
RRNA基因
诊断
cirrhosis
spontaneous peritonitis
16S rRNA gene
PCR
Dot blot
