摘要
目的为了寻找临床病原菌系统，检测和鉴别的有力手段。方法用真细菌保守的１６ＳｒＲＮＡ基因为模板，以ＰＣＲ－Ｄｏｔｂｌｏｔ杂交的方法检测临床病原菌。结果它将１６ＳｒＲＮＡ基因的广谱性和变异性并存之特点和ＰＣＲ－Ｄｏｔｂｌｏｔ杂交的敏感性结合起来，对该法的建立进行了探讨，并初步用于临床感染的检测。结论为细菌通用检测法的建立提供了基础。
Objective To fine the method of defecting clinical pathogens. Method A polymerease chain reaction targeted conserved sequence of eubacterial 16S rRNA geme, combined with dot blot hybridization was used to detect clinical pathogens. Results this method relyed on ubiquity and diversity of 16S rRNA and sensitivity of dot-blot hybridization. Conclusion applied the basis for developing the general diagnostic system for clinical pathogens. ln this paper such a method was developed and applied to diagnose of clinlcal lnfections.
出处
《中华医院感染学杂志》
CAS
CSCD
1998年第1期14-16,共3页
Chinese Journal of Nosocomiology
