摘要
目的:建立酮通颗粒的质量标准。方法:采用薄层色谱(TLC)法鉴别制剂中三七、银杏叶、葛根药材;用高效液相色谱(HPLC)法测定制剂中银杏总黄酮醇苷的含量。结果:TLC斑点清楚,专属性强。槲皮素、山柰素、异鼠李素的检测浓度分别在5.24～83.84、4.56～72.96、2.48～39.68μg·mL-1范围内与各自峰面积积分值呈良好线性关系(r分别为0.9992、0.9991、0.9994);三者平均回收率分别为96.21%、99.14%、95.00%,RSD分别为2.06%、1.19%、1.31%。结论:所建标准可用于酮通颗粒的质量控制。
OBJECTIVE： To establish the quality standard of Tongtong granule. METHODS： Panax notoginseng, Ginkgo biloba and Pueraria lobata were identified qualitatively by TLC and the content of total flavonol glyconsides in G. biloba was determined by HPLC. RESULTS： The TLC spots were distinctive and specific. The linear range of quercetin, kaempferide, isorhamnetin were 5.24- 83.84ug·mL^-1 （r=0.999 2）, 4.56-72.96ug·mL^-1 （r=0.999 1） and 2.48-39.68 ug·mL^-1 （r=0.999 4）, respectively. The average recoveries were 96.21% （RSD = 2.06 % ）, 99.14 % （RSD = 1.19 % ） , 95.00 % （RSD = 1.31% ）, respectively. CONCLUSION ： The method is reliable, accurate, and reproducible for the quality control of Tongtong granule.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第11期1025-1027,共3页
China Pharmacy
