摘要
目的探讨大鼠坐骨神经损伤后内源性脑源性神经营养因子(BDNF)对脊髓前角内生长相关蛋白GAP-43表达的调节。方法大鼠坐骨神经压榨损伤后,腹腔注射BDNF抗体中和内源性BDNF,对照组注射生理盐水,动物存活7d或14d,用Western blot与RT-PCR观察GAP-43在脊髓腰骶膨大部前角的表达。结果与对照组相比,注射BDNF抗体后坐骨神经损伤侧脊髓前角内GAP-43蛋白与其mRNA的表达明显下降,上述改变在统计学上均有显著意义(P<0.01)。结论大鼠坐骨神经损伤后内源性BDNF可能参与脊髓前角内GAP-43表达的调节。
Objective To investigate the effect of endogenous brain derived neurotrophic factor （BDNF） on GAP-43 expression in the anterior horn of the spinal cord in rats following sciatic nerve injury. Methods BDNF antibody was injected intraperitoneally in rats with crushing injury of the sciatic nerve, and the control rats received normal saline only after sciatic nerve injury. At 7 and 14 days after the injection, the expression of GAP-43 in the anterior horn of the corresponding segments of the spinal cord was detected by Western blot and RT-PCR. Results The expressions of GAP-43 protein and mRNA in the anterior horn of the spinal cord were significantly down-regulated in rats with BDNF antibody injection as compared with those in the control group （P0.01）. Conclusion Endogenous BDNF may regulate the expression of GAP-43 in the spinal cord anterior horn after sciatic nerve injury in rats.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2010年第3期569-571,共3页
Journal of Southern Medical University
