摘要
[目的]考察纳豆芽孢杆菌发酵米糠(RBF)提取物的抗衰老作用,为开发米糠资源提供参考。[方法]选用50只健康的昆明小鼠,随机分为5组,即正常对照组、衰老模型组、高剂量组、低剂量组和阳性对照组,正常对照组注射生理盐水,其他各组分别注射相同剂量的D-半乳糖,持续40d,而高剂量组、低剂量组和阳性对照组,在注射的同时分别灌胃RBF、RBF5和VE,测定各组小鼠血清和肝组织中丙二醛(MDA)含量、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性变化。[结果]3个灌胃组小鼠的MDA含量与模型组相比,均有不同程度减少,而SOD和GSH-Px的活性均有不同程度增强,并且灌服高剂量和低剂量的RBF组小鼠的酶活力恢复至正常水平。[结论]RBF有很好的抑制脂质过氧化和增强抗氧化酶活性的作用,适用于生产保健食品。
[Objective] To study anti-aging effect of rice bran extract fermented by Bacillus natto and provide a reference for the development of rice bran resources. [Method] A total of 50 healthy Kunming mice were randomly divided into 5 groups,namely normal control group,aging model group,high-dose group,low-dose group and positive control group. The normal control group was injected with normal saline,but the other groups were injected with the same dose of D-galactose once a day for 40 d. While the high-dose group,low-dose group and positive control group were also given with RBF,RBF5 and VE by gavage after injection. Meanwhile,the malondialdehyde aldehyde （MDA） contents,and the superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） activities in serum and liver tissue of mice were determined in all groups. [Results] The MDA levels of high-dose,low-dose and positive control groups were reduced compared with model group,while the SOD and GSH-Px activities were increased. Moreover,the activities of SOD and GSH-Px in high-dose and low dose groups were returned to normal levels. [Conclusion] RBF can inhibit lipid peroxidation and enhance the activity of peroxidase in vivo,and suitable for the production of health food.
出处
《安徽农业科学》
CAS
北大核心
2010年第9期4788-4789,共2页
Journal of Anhui Agricultural Sciences
基金
信阳师范学院青年骨干教师资助计划项目(2006-014)
关键词
纳豆芽孢杆菌
米糠
发酵
抗衰老
Bacillus natto
Rice bran
Fermentation
Anti-aging
