摘要
[目的]研究羟基喜树碱对人白血病K562细胞增殖与凋亡的影响。[方法]通过光学显微镜、荧光显微镜、电子显微镜、琼脂糖凝胶电泳、流式细胞术等方法,探讨不同浓度羟基喜树碱作用不同时间后,K562细胞凋亡的情况。[结果]羟基喜树碱能够诱导K562细胞凋亡,作用48h的最佳浓度为8.0μg/ml。羟基喜树碱可影响K562细胞增殖周期中的S期,即DNA合成期,细胞在此期停滞,诱导其发生凋亡。[结论]为将羟基喜树碱应用于临床治疗白血病提供前期试验依据。
[Objective]The research aimed to study the effects of campotothecin on multiplication and apoptosis of human leukemic K562 cells. [Method] The multiplication and apoptosis status of K562 cells treated different time by different concentration campotothecin were observed by light microscope,fluorescence microscope,TEM,agrose gel electrophoresis of DNA and flow cytometry. [Result]The results showed that campotothecin could induce K562 cellls apoptosis,and the best concentration was 8.0 μg/ml for 48 h. Campotothecin could affect the S phase among cellular multiplication,cell could be blocked by campotothecin and then apoptosis in this phase. [Conclusion] The study can provide previous experiment basis for campotothecin applying clinical treatments.
出处
《安徽农业科学》
CAS
北大核心
2010年第9期4546-4549,共4页
Journal of Anhui Agricultural Sciences
基金
黑龙江省自然科学基金项目(C200624)
黑龙江省2009年研究生创新科研基金项目(YJSCX2009-106HLJ)
