摘要
目的 :观察三七皂甙R1是否能诱导人白血病细胞株HL 60细胞凋亡及相关基因的变化。方法 :3 0 0～ 1 2 0 0 μg/ml 1三七皂甙R1处理HL 60细胞 ,光镜下观察细胞形态 ;MTT法测细胞生长抑制率 ;流式细胞仪检测凋忘峰。结果 :3 0 0～ 1 2 0 0 μg/ml 1三七皂甙R1可抑制HL 60细胞生长 ,抑制率有剂量依赖性。流式细胞仪检测凋亡峰随药物浓度增大而增加。结论 :三七皂甙R1可抑制HL 60细胞生长 ,诱导其凋亡。
Objective: To observe the apoptosis of human leukemia cell line HL 60 cells induced by sanchinoside R1. Methods: HL 60 cells were treated with 0～1200ug/ml 1 sanchinoside R1 for 3 days and observed under microscope, Rate of growth inhibition was analyzed by MTT. The apoptosis peaks were detected with flow cytometry. Results:sanchinoside R1 remarkably inhibited the growth of HL 60 cells, showing the apoptotic changes in microscope. 300 1200ug.ml 1 sanchinoside R1 could induce HL 60 cells apoptosis and show the apoptosis peaks in flow cytometry. Conclusion:Sanchinoside R1 can induce apoptosis of HL 60 cell.
出处
《四川生理科学杂志》
2004年第1期14-16,共3页
Sichuan Journal of Physiological Sciences
