摘要
[目的]从水浸丹参和三七后药渣中提取丹参酮并确定丹参酮成分。[方法]采用有机溶剂法提取丹参药渣中丹参酮,以薄层层析法检测确定最佳提取溶剂;通过高效液相色谱法确定丹参药渣中丹参酮成分。[结果]乙醚为丹参药渣中提取丹参酮的最佳溶剂。水浸丹参和三七后干药渣,先经乙醇提取得到脂溶性提取物,再以乙醚为溶剂进行索氏提取,丹参酮混合物得率为2.17%;高效液相色谱法检测,丹参酮混合物中丹参酮ⅡA、次甲丹参醌、隐丹参酮、丹参酮I含量分别为3.62%、1.02%、2.56%、2.75%。[结论]丹参药渣中丹参酮成分与药材丹参基本一致,丹参药渣可以作为一种丹参酮资源,具有开发利用的价值。
[Objective] The aim was to extract tanshinone from Salvia miltiorrhiza Bge. dregs and determine tanshinone components. [Method] Organic solvent method was adopted to extract tanshinone from S. miltiorrhiza dregs and TLC was used to determine the optimum extraction solvent. The components of tanshinone was measured with HPLC. [Result] Ether was the best solvent to extract tanshinone from S. miltiorrhiza dregs. After water immersion,dry dregs of S. miltiorrhiza and Panax notoginseng were extracted with ethanol to obtain fat-soluble extracts. Then with ether as the solvent for soxhlet extraction,the yield of crude tanshinone was 2.17%. The HPLC detection showed that the contents of tanshinone ⅡA,methylenetanshinone,cryptotanshinone,tanshinone I were 3.62%,1.02%,2.56%,2.75% respectively. [Conclusion] The components of tanshinone in S. miltiorrhiza dregs were basically the same as tanshinone in medicine S. miltiorrhiza. S. miltiorrhiza dregs could be used as a kind of tanshinone resource,which has the value of development and utilization.
出处
《安徽农业科学》
CAS
北大核心
2010年第9期4539-4541,共3页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金项目(30470055)
辽宁省教育厅创新团队项目(2007T006)
关键词
丹参药渣
丹参酮
提取
Salvia miltiorrhiza Bge. dregs
Tanshinone
Extraction
